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F3422

Sigma-Aldrich

Flt-3/Flk-2 ligand human

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE and HPLC)

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Synonym(s):
FL
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

≤1.0 ng/mL

mol wt

17.6 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... FLT3(2322)

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SRP0349SRP3044H5416
Sigma-Aldrich

F3422

Flt-3/Flk-2 ligand human

Flt3 active human recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

SRP0349

Flt3 active human

Sigma-Aldrich

SRP3044

Flt-3 Ligand human

Sigma-Aldrich

H5416

FLT3 Ligand human

assay

≥98% (SDS-PAGE and HPLC)

assay

≥80% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

activity assay: suitable, inhibition assay: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

biological source

human

biological source

human

biological source

human

biological source

human

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

100

form

lyophilized powder

form

aqueous solution

form

lyophilized

form

lyophilized powder

General description

Mouse and human Flt-3 ligand share ~72% homology and both demonstrate cross-species activity.

Biochem/physiol Actions

Flt-3/Flk-2 Ligand (FL, or Flt-3L) is a transmembrane glycoprotein that binds and activates the Flt-3 receptor. Expression of Flt-3 is primarily restricted among hematopoietic cells to the most primitive progenitor cells.

Physical form

Lyophilized, 0.2 μm sterile filtered, with with 0.5x PBS.

Analysis Note

The biological activity is measured by its ability to stimulate the proliferation of human AML5 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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H G Drexler et al.
Leukemia & lymphoma, 33(1-2), 83-91 (1999-04-08)
FLT3 ligand (FL) acting through its tyrosine kinase receptor FLT3 has pleiotropic and potent effects on hematopoietic cells. The well-described involvement of this ligand-receptor pair in physiological hematopoiesis raised the question whether FL and FLT3 also play a role in
R Namikawa et al.
Stem cells (Dayton, Ohio), 14(4), 388-395 (1996-07-01)
The biological activities of the ligand for the Flk2/Flt3 receptor tyrosine kinase (FL) on human hematopoietic cells are reviewed. In in vitro studies, FL shows relatively few effects by itself on the proliferation and differentiation of hematopoietic cells, but exhibits
S D Lyman et al.
Oncogene, 8(4), 815-822 (1993-04-01)
We have developed rabbit polyclonal antibodies to the C-terminus of the flt3-encoded protein, which is a member of the receptor tyrosine kinase family. Immunoprecipitation using this antiserum brings down two protein bands, a major band of 143 kDa and a
Lyman, S., et al. et al.
The Cytokine Handbook, 727-727 (1998)
O Rosnet et al.
Oncogene, 6(9), 1641-1650 (1991-09-01)
Receptor-type tyrosine kinases presenting an extracellular region with five immunoglobulin-like domains, and strongly related by sequence similarities in the intracellular region, constitute a family of receptors involved in development and function of various cell lineages. We have isolated and characterized

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