F3512

Sigma-Aldrich

Anti-Human IgG (whole molecule)−FITC antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

goat

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

immunofluorescence: 1:32-1:64 using Hep2 cells

conjugate

FITC conjugate

storage temp.

−20°C

Related Categories

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Specificity of anti-human IgG (whole molecule)-FITC antibody for human IgG is determined by immunoelectrophoresis against purified human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. The two heavy chains and two light chains of IgG are connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Immunogen

Pooled normal human serum

Application

Anti-Human IgG (whole molecule)-FITC antibody has been used:
  • in nucleolar staining
  • in single color fluorescent labeling for the detection of lytically induced cells
  • to detect the model antigen
  • in immunohistochemistry
  • in immunofluorescence

Anti-Human IgG (whole molecule)-FITC antibody may be used for anti-nuclear antibody (ANA) assays. The antibody may also be used in FACS-based assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

F. Calabi and M.S. Neuberger
Molecular Genetics of Immunoglobulin (1987)
Ontogeny and distribution of Fc gamma receptors in the human placenta. Transport or immune surveillance?
Bright N A, et al.
Journal of Anatomy, 184(Pt 2), 297-297 (1994)
N A Bright et al.
Journal of anatomy, 184 ( Pt 2), 297-308 (1994-04-01)
The human fetus acquires maternal IgG via the chorioallantoic placenta. Utilising antibodies against 3 characterised subtypes of IgG receptor (Fc gamma R) expressed by human leucocytes, we show by confocal immunofluorescence microscopy that these molecules are also expressed by cells...
Cells lytically infected with Epstein-Barr virus are detected and separable by immunoglobulins from EBV-seropositive individuals
Bhaduri-McIntosh S and Miller G
Journal of Virological Methods, 137(1), 103-114 (2006)
J Cheng et al.
Molecular and cellular biology, 21(18), 6198-6209 (2001-08-18)
Sphingolipids are major components of the plasma membrane of eukaryotic cells and were once thought of merely as structural components of the membrane. We have investigated effects of inhibiting sphingolipid biosynthesis, both in germinating spores and growing hyphae of Aspergillus...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.