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F3651

Sigma-Aldrich

Fluorescein 5(6)-isothiocyanate

≥90% (HPLC)

Synonym(s):
FITC, Fluorescein isothiocyanate
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
Beilstein:
1407295
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥90% (HPLC)

form

powder

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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Fluorescein diacetate used as cell viability stain

Sigma-Aldrich

F7378

Fluorescein diacetate

assay

≥90% (HPLC)

assay

≥90% (HPLC)

assay

-

assay

≥90% (HPLC)

form

powder

form

powder

form

powder

form

-

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

solubility

DMSO: 5 mg/mL

solubility

acetone: 25 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

Fluorescein 5(6)-isothiocyanate has been used:
  • for fluorescence labeling of bacteria
  • as a standard for calibration of fluorometer
  • for the labeling of exosomes
For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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