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F3685

Sigma-Aldrich

Fibroblast Growth Factor-Basic

FGF-Basic, from human, recombinant, expressed in E. coli, carrier free

Synonym(s):
FGB-b, FGF-2, HBGF-2, HBGH-2, Prostatropin, bFGF
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

<1 ng/mL Biological Activity EC50

mol wt

16.0 kDa

packaging

pkg of 4X25 μg
pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

color

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... FGF2(2247)

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This Item
F9786F5542F5392
biological source

human

biological source

human

biological source

human

biological source

bovine pituitary glands

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

assay

≥97% (SDS-PAGE)

assay

-

assay

≥97% (SDS-PAGE)

assay

~90% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

potency

<1 ng/mL Biological Activity EC50

potency

0.03-3.0 ng/mL ED50/EC50

potency

0.287 ng/mL

potency

0.03-3.0 ng/mL ED50/EC50

mol wt

16.0 kDa

mol wt

-

mol wt

15.5 kDa

mol wt

16.4 kDa

General description

Since FGF-Basic is found in a variety of organs, acts on a wide range of cell types, and has multifunctional actions, it has acquired numerous synonyms, including heparin-binding growth factor (class II or beta), eye-derived growth factor I, cartilage-derived growth factor, and astroglial growth factor II5. Purified bovine and human FGF-basic differ by 3 amino acids in sequence3, and are biologically and immunologically cross-reactive.

Citation

1. Gospodarowicz, D., Localization of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature, 249, 123-127 (1974).
2. Gospodarowicz, D. et al., Structural characterization and biological functions of fibroblast growth factor. Endo. Rev., 8, 95-114 (1987).
3. Esch, F. et al., Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc. Natl. Acad. Sci. USA, 82, 6507-6511 (1985).
4. Neufeld, G., and Gospodarowicz, D., Basic and acidic fibroblast growth factors interact with the same cell surface receptors. J. Biol. Chem., 261, 5631-5637 (1986).
5. Lobb, R.R. et al., Purification of heparin-binding growth factors. Anal. Biochem., 154, 1-14 (1986).
6. Abraham, J.A., Human basic fibroblast growth factor: nucleotide sequence and genomic organization. EMBO J., 5, 2523- 2528 (1986).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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