Anti-Human κ Light Chain (bound and free) antibody is obtained from goat antiserum. Immunospecific purification removes all goat serum proteins and immunoglobulins that do not bind to the κ light chain (bound and free). Goat anti-κ light chain is conjugated to fluorescein isothiocyanate (FITC). The antibody preparation is specific for human κ light (human IgA, IgG, and IgM) and free κ light chain (Bence Jones κ myeloma protein). The conjugate shows no reaction with bound or free λ light chains.
Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody is specific for human kappa light chain when tested against purified bound kappa light chain (human IgA, IgG, and IgM) and free kappa light chain (Bence Jones Kappa myeloma protein). The conjugate shows no reaction with bound or free lambda light chains.
Bence Jones-κ myeloma protein
Anti-Human κ Light Chain (Bound and Free)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:16. The antibody was used in flow cytometry analysis in various studies.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Goat polyclonal anti-Human Kappa Light Chain (Bound and Free)−FITC antibody may be used to detect and quantitate the level of kappa light chain containing antibody by fluorescent techniques.
2 mL in glass bottle
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
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