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F4042

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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0.2 MG

$581.00

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$1,310.00

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$2,190.00

$581.00

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About This Item

MDL number:
MFCD00282246
UNSPSC Code:
12352203
NACRES:
NA.32

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M5, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot (chemiluminescent): 10 μg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

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Monoclonal ANTI-FLAG® M5 antibody produced in mouse clone M5, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4042

Monoclonal ANTI-FLAG® M5 antibody produced in mouse

Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

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F3040

Monoclonal ANTI-FLAG® M1 antibody

Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Sigma-Aldrich

A9594

Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal

ANTI-FLAG® BioM2 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® BioM2 antibody, Mouse monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

CY3 conjugate

conjugate

biotin conjugate

clone

M5, monoclonal

clone

M1, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

(Bioreactor)

biological source

mouse

biological source

mouse

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

form

buffered aqueous glycerol solution

General description

Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting[1] and immunofluorescence.[2]

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Preparation Note

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Other Notes

Antibody is not calcium dependent.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
082K91643
082K92041
082K9204
082K91641
052K9158

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