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F4135

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(s):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
MDL number:
eCl@ss:
32160409
NACRES:
NA.71

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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This Item
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Fetal Bovine Serum USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Sigma-Aldrich

F4135

Fetal Bovine Serum

Fetal Bovine Serum New Zealand origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F8067

Fetal Bovine Serum

Fetal Bovine Serum Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

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F8192

Fetal Bovine Serum

Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

F6178

Fetal Bovine Serum

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤25 mg/dL

origin

USA origin

origin

New Zealand origin

origin

Australia origin

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤10 EU/mL endotoxin

impurities

≤25 EU/mL endotoxin

General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
  • as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
  • as a component of blocking medium for treating brainstem slices
  • to improve attachment of dermal explants

Preparation Note

Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
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Articles

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

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