F4512

Sigma-Aldrich

Anti-Human IgG (whole molecule)−FITC antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

conjugate

FITC conjugate

storage temp.

−20°C

Related Categories

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Human IgG (whole molecule)−FITC antibody is specific for human IgG. Rabbit Anti-Human IgG is conjugated to crystalline fluorescein isothiocyanate (FITC) in an alkaline reaction and then further purified to remove free FITC.
IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. FITC (fluorescein isothiocyanate), a fluorochrome dye conjugation is useful in localization of antigen-antibody interaction of biological structures like cells or tissues. Anti-Human IgG (whole molecule) −FITC antibody diluted 1:100 in wash buffer is used in flow cytometry to analyze antibody affinity and virus binding. This antibody can also be used in indirect immunofluorescence antibody test. FITC labeled anti-human IgG antibody reacts specifically with human.

Immunogen

IgG from pooled normal human serum

Application

The antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:64. A minimum working dilution of 1:64 was used on acetone fixed mouse liver cells and anti-nuclear antibody positive serum as the primary antibody. For flow cytometry to label NIH3T3 cells, the antibody was used at a working dilution of 1:100. The antibody was also used in ELISA using human sera.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Anti-Human IgG (whole molecule) −FITC antibody may be used as secondary antibody in indirect immunofluorescence and is also used in flow cytometry .

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Albert Bosch et al.
Methods in molecular biology (Clifton, N.J.), 268, 61-68 (2004-05-25)
Human rotaviruses are considered the main cause of viral gastroenteritis in infants and young children throughout the world. Their transmission is through the fecal-oral route, mostly after ingestion of contaminated water and food. Since an extremely high number of virus...
Evaluation of Serological Tests for the Diagnosis of Visceral Leishmaniasis.
Selcuk kilic, Ayflegul taylan
Turkish Journal of Medical Sciences, 38 (1), 13-19 (2008)
Evaluation of serological tests for the diagnosis of visceral Leishmaniasis
Kilic S et al
Turkish Journal of Medical Sciences, 38, 13-19 (2008)
Liang Wang et al.
Experimental and therapeutic medicine, 8(5), 1473-1478 (2014-10-08)
Heparin is a potent blood anticoagulant that has been demonstrated to attenuate inflammatory responses in sepsis. Sepsis is considered to be a microcirculation-mitochondrial distress syndrome. Azurocidin (AZU), a protein with strong heparin-binding potential that induces inflammatory responses and apoptosis, has...
Detection of Infectious Rotaviruses by Flow Cytometry .
Albert Bosch, Rosa M. Pinto
Springer Semin. Immunopath., 268(2), 61-68 (2004)

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