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F4799

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

3X FLAG® Peptide

Synonym(s):

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

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Pack SizeSKUAvailabilityPrice
4 mg
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$506.00
25 mg
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$2,360.00

About This Item

Empirical Formula (Hill Notation):
C120H169N31O49S
Molecular Weight:
2861.87
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
MFCD01863911

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Product Name

3X FLAG® Peptide, lyophilized powder

assay

≥90% (HPLC/MS)

Quality Level

200

form

lyophilized powder

shipped in

wet ice

storage temp.

2-8°C

General description

Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.
The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG® affinity gels.


Learn more product details in our FLAG® application portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
F3290I2149P1306
3X FLAG® Peptide lyophilized powder

Sigma-Aldrich

F4799

3X FLAG® Peptide

FLAG® Peptide lyophilized powder

Millipore

F3290

FLAG® Peptide

Influenza Hemagglutinin (HA) Peptide ≥97% (HPLC)

Sigma-Aldrich

I2149

Influenza Hemagglutinin (HA) Peptide

Peptide YY human ≥97% (HPLC), synthetic

Sigma-Aldrich

P1306

Peptide YY human

assay

≥90% (HPLC/MS)

assay

≥85% (HPLC)

assay

≥97% (HPLC)

assay

≥97% (HPLC)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

powder

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000469512
0000469513
0000469512
0000469513
0000339717

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Articles

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

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Global Trade Item Number

SKUGTIN
F4799-1MG04061824807516
F4799-4MG04061833617908
F4799-25MG04061833617892
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