F4799

3X FLAG^® Peptide

lyophilized powder

• Synonym(s): Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide
• Empirical Formula (Hill Notation): C₁₂₀H₁₆₉N₃₁O₄₉S
• Molecular Weight: 2861.87
• MDL number: MFCD01863911
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• concentration: (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gel.)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Application

For use in competitive elution of 3X FLAG^® fusion proteins from the ANTI-FLAG^® M2 monoclonal antibody (F1804) in solution or bound to agarose on the ANTI-FLAG^® M2 agarose affinity gel (A2220). This is achieved by Affinity Chromatography. For the gentle elution of 3X FLAG fusion proteins from the ANTI-FLAG^® M2 affinity gels. 1X FLAG Peptide (F3290) will not elute 3X FLAG fusion proteins from ANTI-FLAG^® affinity gels.


Browse additional application references in our FLAG^® Literature portal.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves