Anti-Human IgG (Fab specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

MDL number:

Quality Level

biological source


antibody form

affinity isolated antibody

antibody product type

secondary antibodies




buffered aqueous solution

storage condition

protect from light


immunofluorescence: 1:16-1:32 using Hep2 cells


FITC conjugate

storage temp.


Related Categories

General description

The product binds all human Igs and provides reduced background with mouse or rat samples.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.


Anti-Human IgG (Fab specific)-FITC antibody has been used:
  • in fluorescent staining
  • in dual color fluorescent labeling
  • in fluorescence-activated cell sorting (FACS) analysis

Anti-Human IgG (Fab specific)-FITC antibody produced in goat may be used at a working antibody dilution of 1:16 - 1:32 for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. This antibody was used in FACS of HNE2 and KNE2/LMP1 cells. It was also used to perform TARDIS assay for detection of topoisomerases I and II that are covalently bound to DNA in K562 leukemia cells.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Human IgG (Fab specific) antibody allows for the visualization of protein by fluorescent microscopy.

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Certificate of Analysis

Certificate of Origin

The Immunoglobulins: Structure and Function (1998)
Tomoyuki Mutoh et al.
Nature communications, 11(1), 1253-1253 (2020-03-11)
The presence of antiendothelial cell antibodies (AECAs) has been documented in Takayasu arteritis (TAK), a chronic granulomatous vasculitis. Here, we identify cell-surface autoantigens using an expression cloning system. A cDNA library of endothelial cells is retrovirally transfected into a rat...
Dawei Zhang et al.
Viruses, 5(4), 1131-1142 (2013-04-24)
Latent Membrane Protein 1 (LMP1) is a primary target for controlling tumorigenesis in Epstein-Barr virus related malignancies; in this study, we aimed to develop a specific antibody against the TES1 domain of the oncogenic LMP1. We screened a full human...
Molecular Genetics of Immunoglobulin (1987)
In vitro efficacy of immuno-chemotherapy with anti-EGFR human Fab-Taxol conjugate on A431 epidermoid carcinoma cells.
Wang, et al.
Cancer Biology & Therapy, 6, 980-987 (2020)

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