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F5554

Sigma-Aldrich

Formalin, 10%, Neutral Buffered, Wintergreen Scented

histological fixative, pH 6.9-7.1

Synonym(s):

Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

Quality Level

shelf life

Expiry date on the label.

color

colorless

pH

6.90-7.10

application(s)

hematology
histology

storage temp.

room temp

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Application

Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

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Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
p38alpha MAP kinase as a sensor of reactive oxygen species in tumorigenesis
Dolado I, et al.
Cancer Cell, 11(2), 191-205 (2007)
Neither epithelial nor mesenchymal circulating tumor cells isolated from breast cancer patients are tumorigenic in NOD-scid Il2rg null mice
Donnenberg VS, et al.
NPJ breast cancer, 2, 16004-16004 (2016)
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Magdeldin, et al.
Proteomics, 12(7), 1045-1058 (2012)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)

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