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F6162

Sigma-Aldrich

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal

clone FB-8, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse, Anti-FGF2
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FB-8, monoclonal

form

buffered aqueous solution

mol wt

antigen 18 kDa

species reactivity

bovine, human

technique(s)

dot blot: suitable
indirect ELISA: suitable
neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FGF2(2247)

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

fractionated antiserum

clone

FB-8, monoclonal

clone

FG9-77, monoclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized powder

mol wt

antigen 18 kDa

mol wt

antigen 25 kDa

mol wt

antigen 115-125 kDa (doublet)

mol wt

-

General description

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa.

Specificity

The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1.

Immunogen

recombinant human FGF2.

Application

Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in:
  • in immunoblotting
  • in enzyme linked immunosorbent assay (ELISA)
  • in dot blot
  • in radio immune assay (RIA)
  • in neutralization bioassays

Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture.

Biochem/physiol Actions

Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Eric Sulpice et al.
Blood, 100(9), 3087-3094 (2002-10-18)
Platelet factor 4 (PF-4) is a member of the chemokine family with powerful antiangiogenic properties. The mechanism by which PF-4 inhibits endothelial cell proliferation is unclear. We investigated the effects of PF-4 on the intracellular signal transduction induced by basic
M Arras et al.
The Journal of clinical investigation, 101(1), 40-50 (1998-02-14)
We have previously shown that monocytes adhere to the vascular wall during collateral vessel growth (arteriogenesis) and capillary sprouting (angiogenesis). In this study we investigated the association of monocyte accumulation with both the production of the cytokines-basic fibroblast growth factor
Ultrastructural immunolocalization of basic fibroblast growth factor in fibroblasts and extracellular matrix
Aktas RG and Kayton RJ
Histochemistry and Cell Biology, 113(3), 227-233 (2000)
An intrinsic quality-control mechanism ensures unconventional secretion of fibroblast growth factor 2 in a folded conformation
Torrado L C,et al.
Journal of Cell Science, 122(18), 3322-3329 (2009)
B P Mari et al.
The Journal of biological chemistry, 273(1), 618-626 (1998-02-07)
Stromelysin-3 (STR-3) is a recently characterized matrix metalloproteinase (MMP) with a unique pattern of expression and substrate specificity. Unlike other MMPs, STR-3 is consistently and dramatically overexpressed by multiple epithelial malignancies, including carcinomas of the breast, lung, colon, head and

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