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F6926

Sigma-Aldrich

Fibroblast Growth Factor-8b from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

FGF-8b

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

15-60 ng/mL

mol wt

calculated mol wt 23.6 kDa
predicted mol wt 22 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Fgf8(14179)

General description

Fibroblast growth factor 8 (FGF8) is a member of the FGF family, and was initially cloned from androgen-dependent mouse mammary carcinoma cell line. Alternative splicing of this gene produces four different isoforms mainly, FGF8a, FGF8b, FGF8e and FGF8f.

Application

Fibroblast Growth Factor-8b from mouse has been used-
  • for subgerminal cavity injection in fertilized quail, chicken, turtle and emu eggs
  • in the induction of neural differentiation in stem cells from Human Exfoliated Deciduous teeth (SHED) and
  • in experiments conducted on fertilized hens′ eggs, where FGF8 induced SOX3 gene.

Biochem/physiol Actions

FGF-8b is involved in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system. FGF-8b activates the "c" splice form of FGFR-2, FGFR-3, and FGFR-4.
Fibroblast growth factor 8b (FGF8b) isoform of FGF8 has the most potent angiogenic and tumorigenic properties. In prostate cancer, it is responsible for angiogenesis, tumorigenesis and invasion. Exogenous expression of this gene in prostate epithelium leads to prostatic intraepithelial neoplasia (PIN). It also interacts with pattern recognition receptor long pentraxin-3 (PTX3), which is involved in local inflammation.

Physical form

Lyophilized from a 0.2μm filtered solution in 20mM MOPS, 0.1M sodium sulfate, 0.02% Brij-35, pH 7 containing 50ug of BSA per 1μg as a carrier protein

Analysis Note

The biological activity is measured by its ability to stimulate proliferation of NR6R-3T3 fibroblasts.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Hyung Chul Lee et al.
Development genes and evolution, 232(5-6), 115-123 (2022-09-24)
During primitive streak formation in the chick embryo, cells undergo mesendoderm specification and convergent extension at the same time and in the same cells. Previous work has implicated cVG1 (GDF3) as a key factor for induction of primitive streak identity
Claudia Linker et al.
Development (Cambridge, England), 131(22), 5671-5681 (2004-10-29)
A dominant molecular explanation for neural induction is the 'default model', which proposes that the ectoderm is pre-programmed towards a neural fate, but is normally inhibited by endogenous BMPs. Although there is strong evidence favouring this in Xenopus, data from
Cantas Alev et al.
Development (Cambridge, England), 140(13), 2691-2696 (2013-05-24)
Mesoderm is formed during gastrulation. This process takes place at the blastopore in lower vertebrates and in the primitive streak (streak) in amniotes. The evolutionary relationship between the blastopore and the streak is unresolved, and the morphogenetic and molecular changes
Abolghasem Esmaeili et al.
Avicenna journal of medical biotechnology, 6(1), 21-26 (2014-02-20)
Stem cells from Human Exfoliated Deciduous teeth (SHED) have the capability to differentiate into neural cells. Neurotrophins including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) have neurogenesis, neurotrophic, or neuroprotective effects and are expressed
Shona Pfeiffer et al.
Cell biology international, 34(9), 959-967 (2010-04-15)
Amniotic fluid is known to yield a number of cell types which are multipotent, ethically derived, genetically stable, easily grown, expanded and possess favourable immunogenicity, which has resulted in an increasing interest for use in various neuronal disorders such as

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