MilliporeSigma
All Photos(1)

Documents

F6926

Sigma-Aldrich

Fibroblast Growth Factor-8b from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

All Photos(1)
Synonym(s):
FGF-8b
MDL number:
MFCD01323276
NACRES:
NA.32

biological source

mouse

Quality Level

100

recombinant

expressed in E. coli

Assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

15-60 ng/mL

mol wt

calculated mol wt 23.6 kDa
predicted mol wt 22 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

UniProt accession no.

P37237

storage temp.

−20°C

Gene Information

mouse ... Fgf8(14179)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F7301F4537F5542
Fibroblast Growth Factor-8b from mouse >97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Sigma-Aldrich

F6926

Fibroblast Growth Factor-8b from mouse

Sigma-Aldrich

Sigma-Aldrich

F7301

Fibroblast Growth Factor-18 human

Sigma-Aldrich

Sigma-Aldrich

F4537

Fibroblast Growth Factor-5 human

Sigma-Aldrich

Sigma-Aldrich

F5542

Fibroblast Growth Factor-Acidic human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

assay

>95% (SDS-PAGE and HPLC)

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

calculated mol wt 23.6 kDa, predicted mol wt 22 kDa

mol wt

predicted mol wt 21.2 kDa

mol wt

27 kDa

mol wt

15.5 kDa

packaging

pkg of 25 μg

packaging

pkg of 25 μg

packaging

pkg of 50 μg

packaging

pkg of 25 μg

General description

Fibroblast growth factor 8 (FGF8) is a member of the FGF family, and was initially cloned from androgen-dependent mouse mammary carcinoma cell line. Alternative splicing of this gene produces four different isoforms mainly, FGF8a, FGF8b, FGF8e and FGF8f.

Application

Fibroblast Growth Factor-8b from mouse has been used-
  • for subgerminal cavity injection in fertilized quail, chicken, turtle and emu eggs
  • in the induction of neural differentiation in stem cells from Human Exfoliated Deciduous teeth (SHED) and
  • in experiments conducted on fertilized hens′ eggs, where FGF8 induced SOX3 gene.

Biochem/physiol Actions

FGF-8b is involved in ectodermal differentiation in the post-gastrulation embryo, including outgrowth and patterning of the face, limbs, and central nervous system. FGF-8b activates the "c" splice form of FGFR-2, FGFR-3, and FGFR-4.
Fibroblast growth factor 8b (FGF8b) isoform of FGF8 has the most potent angiogenic and tumorigenic properties. In prostate cancer, it is responsible for angiogenesis, tumorigenesis and invasion. Exogenous expression of this gene in prostate epithelium leads to prostatic intraepithelial neoplasia (PIN). It also interacts with pattern recognition receptor long pentraxin-3 (PTX3), which is involved in local inflammation.

Physical form

Lyophilized from a 0.2μm filtered solution in 20mM MOPS, 0.1M sodium sulfate, 0.02% Brij-35, pH 7 containing 50ug of BSA per 1μg as a carrier protein

Analysis Note

The biological activity is measured by its ability to stimulate proliferation of NR6R-3T3 fibroblasts.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315 - H319

Precautionary Statements

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Cantas Alev et al.
Development (Cambridge, England), 140(13), 2691-2696 (2013-05-24)
Mesoderm is formed during gastrulation. This process takes place at the blastopore in lower vertebrates and in the primitive streak (streak) in amniotes. The evolutionary relationship between the blastopore and the streak is unresolved, and the morphogenetic and molecular changes
Claudia Linker et al.
Development (Cambridge, England), 131(22), 5671-5681 (2004-10-29)
A dominant molecular explanation for neural induction is the 'default model', which proposes that the ectoderm is pre-programmed towards a neural fate, but is normally inhibited by endogenous BMPs. Although there is strong evidence favouring this in Xenopus, data from
Hyung Chul Lee et al.
Development genes and evolution, 232(5-6), 115-123 (2022-09-24)
During primitive streak formation in the chick embryo, cells undergo mesendoderm specification and convergent extension at the same time and in the same cells. Previous work has implicated cVG1 (GDF3) as a key factor for induction of primitive streak identity
Abolghasem Esmaeili et al.
Avicenna journal of medical biotechnology, 6(1), 21-26 (2014-02-20)
Stem cells from Human Exfoliated Deciduous teeth (SHED) have the capability to differentiate into neural cells. Neurotrophins including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) have neurogenesis, neurotrophic, or neuroprotective effects and are expressed
Shona Pfeiffer et al.
Cell biology international, 34(9), 959-967 (2010-04-15)
Amniotic fluid is known to yield a number of cell types which are multipotent, ethically derived, genetically stable, easily grown, expanded and possess favourable immunogenicity, which has resulted in an increasing interest for use in various neuronal disorders such as
View All Related Papers

Articles

Fibroblast Growth Factor Family (FGF)

Fibroblast growth factors in cell culture and various growth factors for your research

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service