MilliporeSigma
All Photos(1)

Documents

F7296

Sigma-Aldrich

Fructosyl-Amino Acid Oxidase from Corynebacterium sp.

recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein

All Photos(1)
Enzyme Commission number:
1.5.3.x
EC Number:
232-914-2
MDL number:
MFCD03456889

recombinant

expressed in E. coli

Quality Level

200

form

lyophilized powder

specific activity

≥0.45 units/mg protein

mol wt

~88 kDa by electrophoresis

storage temp.

−20°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G3512SAE0051SAE0044
Fructosyl-Amino Acid Oxidase from Corynebacterium sp. recombinant, expressed in E. coli, lyophilized powder, ≥0.45 units/mg protein

Sigma-Aldrich

F7296

Fructosyl-Amino Acid Oxidase from Corynebacterium sp.

Glycerol Dehydrogenase from Cellulomonas sp. lyophilized powder, 50-125 units/mg protein

Sigma-Aldrich

G3512

Glycerol Dehydrogenase from Cellulomonas sp.

Sigma-Aldrich

Sigma-Aldrich

SAE0051

Glycerol 3-phosphate Oxidase from Enterococcus sp.

Choline Oxidase from Arthrobacter sp. recombinant, expressed in E. coli

Sigma-Aldrich

SAE0044

Choline Oxidase from Arthrobacter sp.

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

specific activity

≥0.45 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥35 unit/mg solid

specific activity

≥12 units/mg protein

mol wt

~88 kDa by electrophoresis

mol wt

~390 kDa

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Fructosyl amino acid oxidase [fructosyl-a-l-amino acid:oxygen oxidoreductase] is a flavoprotein that catalyzes the oxidation of fructosyl amino acids to form glucosone, amino acid and hydrogen peroxide.
Enzyme Commission (E.C.) 1.5.3.x

Application

Fructosyl-Amino Acid Oxidase from Corynebacterium sp has been used in glycated haemoglobin HbA1c detection in blood samples using quartz crystal microbalance (QCM) based detection.
Fructosyl-amino acid oxidase can be used to detect the levels of glycated proteins, which are markers for diabetes mellitus.

Biochem/physiol Actions

Fructosamines are formed when glucose is condensed amino group of amino acids or proteins. Fructosamine oxidases (FAOX) catalyze the oxidative deglycation of low molecular weight fructosamines. Fructosyl amino acid oxidase catalyzes the oxidation of the C-N bond linking the C1 of the fructosyl moiety and the nitrogen of the amino group of fructosyl amino acids.
Fructosyl-Amino Acid Oxidase (FAOD) comprises of FAD-binding motifs and is classified into three types based on substrate specificity. The engineered Corynebacterium Fructosyl-Amino Acid Oxidase is stable at 45°C and could be exploited for the development of glycated protein biosensing system and glycated hemoglobin HbA1c measurements. FAOD shares sequence homology with fructosyl peptide oxidase and both are effective on α-fructosyl substrates.
Suitable for the determination of fructosyl-L-amino acid.

Unit Definition

One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Dess-Martin periodinane 97%

Sigma-Aldrich

274623

Dess-Martin periodinane

Peroxidase from horseradish Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)

Sigma-Aldrich

P8375

Peroxidase from horseradish

Red Blood Cell Lysis Buffer solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Roche

11814389001

Red Blood Cell Lysis Buffer

Hemoglobin human lyophilized powder

Sigma-Aldrich

H7379

Hemoglobin human

Structural basis of the substrate specificity of the FPOD/FAOD family revealed by fructosyl peptide oxidase from Eupenicillium terrenum
Gan W, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 71(4), 381-387 (2015)
C Gerhardinger et al.
The Journal of biological chemistry, 270(1), 218-224 (1995-01-06)
A Pseudomonas sp. soil strain, selected for its ability to grow on epsilon-(1-deoxyfructosyl) aminocaproic acid, was induced to express a membrane-bound enzymatic activity which oxidatively degrades Amadori products into free fructosamine. Apparent Km values for fructosyl aminocaproate, epsilon-fructosyl lysine, fructosyl
A quartz crystal microbalance-based biosensor for enzymatic detection of hemoglobin A1c in whole blood
Park HJ and Lee SS
Sensors and Actuators B, Chemical, 258, 836-840 (2018)
R Romero Campos et al.
Angiologia, 43(3), 130-131 (1991-05-01)
Authors report the case of a young man affected by an aneurysm at the humeral artery. The relevance of this case was due to its notable rare incidence at such level as well as its difficult etiologic diagnosis. Patient was
S A Schellini et al.
Acta ophthalmologica, 67(5), 601-604 (1989-10-01)
The authors report a case of fibrohistiocytoma of the limbus and discuss the clinical, histopathological and immunohistochemical findings concerning this type of lesion, with a comparison of their findings with those reported in the literature.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service