F7378

Fluorescein diacetate

Powder

• Synonym(s): 3,6-Diacetoxyfluoran, Di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₄H₁₆O₇
• CAS Number: 596-09-8
• Molecular Weight: 416.38
• Beilstein/REAXYS Number: 62575
• EC Number: 209-877-6
• MDL number: MFCD00005062
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894939
• NACRES: NA.47

Properties

• Product Name: Fluorescein diacetate, used as cell viability stain
• Quality Level: 200
• form: powder
• technique(s): titration: suitable
• color: white to faint yellow
• mp: 200-203 °C (lit.)
• solubility: acetone: 25 mg/mL
• ε (extinction coefficient): ≥86000 at 488-494 nm in 0.1 M NaOH
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
• InChI key: CHADEQDQBURGHL-UHFFFAOYSA-N

Description

General description

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.[1]

Lipase substrate

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.[2][3]

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)