All Photos(2)

F7387

Sigma-Aldrich

Monoclonal Anti-Fibronectin antibody produced in mouse

clone FN-15, ascites fluid

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FN-15, monoclonal

contains

15 mM sodium azide

species reactivity

mouse, human, chicken

technique(s)

indirect ELISA: 1:5,000
indirect immunofluorescence: 1:200 using cultured human fibroblasts
microarray: suitable
western blot: 1:800

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)

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General description

Monoclonal Anti-Fibronectin (mouse IgG1 isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin (FN) belongs to glycoprotein family widely expressed in many tissues. FN is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. There are three isoforms of fibronectin, that includes cellular FN, plasma FN and fetal FN. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN gene in human chromosome is mapped to 2q35.
Mouse monoclonal clone FN-15 anti-Fibronectin antibody labels fibronectin in the pericellular extracellular matrix of cultured human fibroblasts using indirect immunofluorescent methods. It also labels fibronectin in chicken and mouse cells. The FN-15 antibody specifically stains fibronectin in immunoblotting, and in an ELISA is reactive with human plasma fibronectin.
Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN′s play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.

Immunogen

fibronectin from human plasma

Application

Mouse monoclonal clone FN-15 anti-Fibronectin antibody may be used to specifically localize fibronectin in human cell culture and in tissue sections by immunoblotting and immunohistochemical techniques. Monoclonal antibodies reacting specifically with FN may be used to localize FN in human cell cultures, in tissue sections and in plasma.
Monoclonal Anti-Fibronectin may be used to specifically localize fibronectin in human cell culture and in tissue sections by immunoblotting, immunohistochemical techniques and to stain fibronectin in hydrogels.

Biochem/physiol Actions

Fibronectin (FN) play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells. FN is upregulated in many cancers. FN increases the expression of matrix metalloproteinases (MMPs), promoting cancer cell invasion and metastasis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Ehab El-Hallous et al.
The Journal of biological chemistry, 282(12), 8935-8946 (2007-01-27)
Fibrillin-containing microfibrils in elastic and nonelastic extracellular matrices play important structural and functional roles in various tissues, including blood vessels, lung, skin, and bone. Microfibrils are supramolecular aggregates of several protein and nonprotein components. Recently, a large region in the
High expression of Fibronectin 1 suppresses apoptosis through the NF-kappaB pathway and is associated with migration in nasopharyngeal carcinoma
Wang J, et al.
American Journal of Translational Research, 9(10), 4502-4502 (2017)
Deletion of diacylglycerol-responsive TRPC genes attenuates diabetic nephropathy by inhibiting activation of the TGFbeta1 signaling pathway
Liu B, et al.
American Journal of Translational Research, 9(12), 5619-5619 (2017)
Intracellular signaling cascades triggered by the NK1 fragment of hepatocyte growth factor in human prostate epithelial cell line PNT1A
Pavone LM, et al.
Cellular Signalling, 23(12), 1961-1971 (2011)
C L Gladson et al.
The Journal of clinical investigation, 88(6), 1924-1932 (1991-12-01)
Glioblastoma multiforme, the most malignant astroglial-derived tumor, grows as an adherent mass and locally invades normal brain. An examination of adult cerebral glioblastoma biopsy material for the expression of adhesive proteins that might potentiate adhesion and invasion demonstrated tumor cell-associated

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