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  • F8053
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F8053

Sigma-Aldrich

Anti-FADD antibody, Mouse monoclonal

clone FD19, purified from hybridoma cell culture

Synonym(s):
Anti-Fas Associated Death Domain
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FD19, monoclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of A431 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FADD(8772)

General description

Anti-FADD antibody, Mouse monoclonal (mouse IgG1) is derived from the FD19 hybridoma produced by the fusion of murine myeloma cells (NS1) and splenocytes from mouse immunized with purified recombinant human FADD.

Immunogen

purified recombinant human FADD.

Application

Anti-FADD antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • enzyme-linked immunosorbent assay (ELISA)

Packaging

200 μL in glass insert

Biochem/physiol Actions

FADD (Fas Associated Death Domain) is associated with various non-apoptotic cellular pathways such as regulation of cell cycle machinery in T lymphocytes connected to the phosphorylation state of FADD and to the FasL/TRAIL-induced transcriptional activation of c-fos protooncogene. It also interacts with the hepatitis C virus core protein in the HEK-293 cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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