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F8053

Sigma-Aldrich

Anti-FADD antibody, Mouse monoclonal

clone FD19, purified from hybridoma cell culture

Synonym(s):
Anti-Fas Associated Death Domain
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FD19, monoclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

human

concentration

~2 mg/mL

application(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of A431 cells

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FADD(8772)

General description

Anti-FADD antibody, Mouse monoclonal (mouse IgG1) is derived from the FD19 hybridoma produced by the fusion of murine myeloma cells (NS1) and splenocytes from mouse immunized with purified recombinant human FADD.

Immunogen

purified recombinant human FADD.

Application

Anti-FADD antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • enzyme-linked immunosorbent assay (ELISA)

Packaging

200 μL in glass insert

Biochem/physiol Actions

FADD (Fas Associated Death Domain) is associated with various non-apoptotic cellular pathways such as regulation of cell cycle machinery in T lymphocytes connected to the phosphorylation state of FADD and to the FasL/TRAIL-induced transcriptional activation of c-fos protooncogene. It also interacts with the hepatitis C virus core protein in the HEK-293 cell line.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Qinghong Kong et al.
International journal of molecular sciences, 19(10) (2018-10-03)
Naphthyridine derivatives are a widely-used class of heterocycles due to their pharmacological activities. A novel compound (10-Methoxy-1,2,3,4-tetrahydrobenzo(g)(1,3) diazepino(1,2-a)-(1,8)naphthyridin-6-yl)(phenyl) methanone (named 3u), showed good anticancer activity in the human malignant melanoma cell line A375 via Thiazolyl Blue Tetrazolium Bromide (MTT) assay....
Ying Huang et al.
International journal of oncology, 49(1), 153-163 (2016-05-24)
The discovery of the TRAIL protein and its death receptors DR4/5 changed the horizon of cancer research because TRAIL specifically kills cancer cells. However, the validity of TRAIL-based cancer therapies has yet to be established, as most cancer cells are...
Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity
Graves JD, et al.
Cancer Cell, 26(2), 177-189 (2014)
Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast
Choi EJ, et al.
World Journal of Surgical Oncology, 12(1), 137-137 (2014)
Overcoming resistance to TRAIL-induced apoptosis in solid tumor cells by simultaneously targeting death receptors, c-FLIP and IAPs
Huang Y, et al.
International Journal of Oncology, 49(1), 153-163 (2016)

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