F8424

Sigma-Aldrich

Fibroblast Growth Factor-4 human

FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):
hFGF-4, FGF-4, K-FGF
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

≤10.0 ng/mL

quality

endotoxin tested

mol wt

19 kDa

packaging

pkg of 25 and 100 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... FGF4(2249), FGF4(2249)

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General description

Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins. Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface. There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids. The FGF4 gene is mapped to human chromosome 11q13.3.

Application

Fibroblast Growth Factor-4 human has been used:
  • To maintain trophectoderm stem cells.
  • In neurogenic differentiation.
  • In hepatocyte differentiation.

Biochem/physiol Actions

Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.
Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.

Physical form

Lyophilized from a 0.2 μm filtered buffer solution containing a carrier protein.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of thymidine uptake using BaF3 cells expressing FGF receptors.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

The oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells
Shi H, et al.
Biochemical and biophysical research communications, 471(1), 89-94 (2016)
Derivation, maintenance, and characterization of rat embryonic stem cells in vitro
Embryonic Stem Cell Protocols, 45-58 (2006)
Fibroblast growth factor signalling: from development to cancer
Turner N and Grose R
Nature Reviews. Cancer, 10(2), 116-116 (2010)
Endometrial regenerative cells: a novel stem cell population
Meng X, et al.
Journal of Translational Medicine, 5(1), 57-57 (2007)
Cellular signaling by fibroblast growth factor receptors
Eswarakumar VP, et al.
Cytokine & Growth Factor Reviews, 16(2), 139-149 (2005)
Articles
Fibroblast growth factors in cell culture and various growth factors for your research
Read More

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