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F9259

Sigma-Aldrich

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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MDL number:
MFCD00162659
NACRES:
NA.46

biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:128

storage temp.

2-8°C

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F9259

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

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F8264

Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat

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F9384

Anti-Mouse IgA (α-chain specific)−FITC antibody produced in goat

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F5384

Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:128

technique(s)

direct immunofluorescence: 1:64

technique(s)

direct immunofluorescence: 1:16

technique(s)

direct immunofluorescence: 1:32

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Specificity

Binds mouse IgM only; does not bind other mouse Igs.

Immunogen

Purified mouse IgM

Application

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Chenjie Zhou et al.
Hematology (Amsterdam, Netherlands), 22(2), 119-127 (2016-07-16)
Monoclonal anti-human blood group A (51A8) and B (63B6) antibody reagents were prepared using the serum-free technique. The aims of this research were to characterize the serum-free reagents and prove their reliabilities in routine use. Experiments including antigen-antibody agglutination testing
F Carreiras et al.
International journal of cancer, 63(4), 530-536 (1995-11-15)
Accumulating evidence suggests that integrins, which participate in many complex cellular processes, are important for tumor progression and metastasis. In order to understand the role of these cell-surface receptors and of their ligands in the biological behavior of ovarian tumor
Bone marrow stromal cells improve cardiac performance in healed infarcted rat hearts
Olivares EL et al
American Journal of Physiology. Heart and Circulatory Physiology, 287, H646-H670 (2004)
Joanna R Morris et al.
Human molecular genetics, 13(8), 807-817 (2004-02-21)
The N-terminus of the BRCA1 protein bears a RING finger domain that functions as an E3 ubiquitin ligase in vitro where it is able to catalyse the synthesis of monoubiquitin and polyubiquitin targeted proteins. This activity is greatly increased when
Musarrat Maisha Reza et al.
Oncotarget, 8(58), 98553-98566 (2017-12-13)
Irisin is an exercise induced myokine that is shown to promote browning of adipose tissue and hence, increase energy expenditure. Furthermore, our unpublished results indicate that Irisin improves myogenic differentiation and induces skeletal muscle hypertrophy. Since exercise induced skeletal muscle
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