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F9378

Sigma-Aldrich

Ficoll® 400

Type 400-DL, lyophilized powder

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Synonym(s):
Polysucrose 400
CAS Number:
MDL number:

biological source

sugar beets (Beta vulgaris)

Quality Level

type

Type 400-DL

form

lyophilized powder

color

white

solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

storage temp.

15-25°C

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1 of 4

This Item
F1418F8016F2637
Ficoll® 400 Type 400-DL, lyophilized powder

F9378

Ficoll® 400

-
Ficoll® 400 BioXtra, Type 400-DL, lyophilized powder

F1418

Ficoll® 400

Premium Grade
Ficoll® 400 lyophilized powder, Type 400-DL, BioReagent, suitable for cell culture

F8016

Ficoll® 400

-
Sigma-Aldrich

F2637

Ficoll® 400

-
solubility

H2O: soluble 100 mg/mL, clear to slightly hazy, colorless to faintly yellow

solubility

water: soluble

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

lyophilized powder

form

lyophilized powder

form

-

form

lyophilized powder

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

storage temp.

room temp

color

white

color

white

color

-

color

-

Application

Ficoll® 400 is used together with sodium diatrizoate (Paque) to create Ficoll-Paque, a density mixture, used for the separation of cells and organelles, especially blood derived lymphocytes and monocytes. Ficoll 400 may be used for the development of new cell and organelle separation systems. Ficoll 400 is used in tissue engineering and macromolecular conformation research for the development, evaluation and use of macromolecular crowding (MMC) systems and configurations.
Used for cell separation and organelle isolation.

Other Notes

A nonionic synthetic polymer of sucrose.
Dialyzed
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Histopaque®-1077 sterile-filtered, density: 1.077 g/mL

Sigma-Aldrich

10771

Histopaque®-1077

Ficoll® PM 70 Cytiva 17-0310-10, pack of 100 g

GE17-0310-10

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Ficoll® PM 70 Cytiva 17-0310-50, pack of 500 g

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Ficoll® PM 70

Ficoll®-Paque PREMIUM 1.084 Cytiva 17-5446-02, pack of 6 × 100 mL

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Ficoll®-Paque PREMIUM 1.084

Ficoll® Paque Plus Cytiva 17-1440-03, pack of 6 × 500 mL

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Mirna González-González et al.
Biotechnology progress, 30(3), 700-707 (2014-01-23)
Aqueous two-phase systems (ATPS) represent a promising strategy for the recovery of CD133(+) stem cells. This particular type of stem cells has great potential for research and clinical applications. Traditional [polyethylene glycol (PEG), dextran (DEX), and ficoll] and novel (Ucon)
Lucie Kosinova et al.
Islets, 11(6), 129-140 (2019-09-10)
Instant Blood-Mediated Inflammatory Reaction (IBMIR) is a major cause of graft loss during pancreatic islet transplantation, leading to a low efficiency of this treatment method and significantly limiting its broader clinical use. Within the procedure, transplanted islets obstruct intrahepatic portal
Yanyan Xu et al.
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 24(4), 406-409 (2010-05-13)
The purity and activity of islets will greatly affect the outcome of xenotransplantation therapy of type 1 diabetes mellitus. To set up an improved method of the isolation and purification of rat islets, which can obtain high-purity, high-yield, and high-viability
Bo Chen et al.
Tissue engineering. Part C, Methods, 19(8), 586-595 (2013-02-21)
Macromolecular crowding (MMC) has been shown to have a beneficial effect on production and maturation of extracellular matrices in a monolayer cell culture model. To explore its potential in tissue engineering, a mixture of Ficoll 70 and Ficoll 400 is
Frank Brand et al.
Acta neuropathologica, 139(1), 175-192 (2019-09-02)
In search of novel genes associated with glioma pathogenesis, we have previously shown frequent deletions of the KIAA1797/FOCAD gene in malignant gliomas, and a tumor suppressor function of the encoded focadhesin impacting proliferation and migration of glioma cells in vitro

Protocols

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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