F9512

Sigma-Aldrich

Anti-Human IgG (Fc specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti Human Igg Fitc, Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat
MDL number:
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

conjugate

FITC conjugate

storage temp.

−20°C

Related Categories

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG.

Specificity

Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples

Immunogen

Purified human IgG Fc fragment

Application

Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in:
  • immunofluorescence
  • immunohistochemistry
  • surface labeling experiments

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense.

Other Notes

No cross-reaction with mouse and rat IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Certificate of Analysis
Certificate of Origin
T N Williams et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 56(2), 96-103 (2003-11-11)
The acquisition of antibodies directed toward variant surface antigens (VSAs) expressed on the surface of the trophozoite-infected red blood cell is an important determinant of natural immunity to Plasmodium falciparum malaria. In recent years, flow cytometry has been used increasingly...
Usha Bughani et al.
PloS one, 12(7), e0180088-e0180088 (2017-07-04)
CD6 is associated with T-cell modulation and is implicated in several autoimmune diseases. We previously demonstrated that Itolizumab, a CD6 domain 1 (CD6D1) specific humanized monoclonal antibody, inhibited the proliferation and cytokine production by T lymphocytes stimulated with anti-CD3 antibody...
Edward Littleton et al.
Molecular & cellular proteomics : MCP, 8(7), 1688-1696 (2009-04-01)
There is increasing interest in the role of antibodies targeting specific membrane proteins in neurological and other diseases. The target(s) of these pathogenic antibodies is known in a few diseases, usually when candidate cell surface proteins have been tested. Approaches...
Towards translational ImmunoPET/MR imaging of invasive pulmonary aspergillosis: the humanised monoclonal antibody JF5 detects Aspergillus lung infections in vivo
Davies G, et al.
Theranostics, 7(14), 3398-3398 (2017)
Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-kappaB signaling pathway
Wang L, et al.
Oncology Letters, 12(6), 4411-4418 (2016)
Articles
Antibody-based serology tests are useful in identifying subjects with an adaptive immune response to the SARS-CoV-2 virus. Anti-human immunoglobulin antibodies allow for quick and simple, yet reliable assays with easy readouts and can also be adapted for high-throughput screening.
Read More

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