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F9786
Fibroblast Growth Factor-Basic Heparin Stabilized human
recombinant, expressed in E. coli, aqueous solution, suitable for cell culture
Synonym(s):
rhbFGF
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Product Name
Fibroblast Growth Factor-Basic Heparin Stabilized human, FGF-Basic, recombinant, expressed in E. coli, sterile-filtered, aqueous solution, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
sterility
sterile-filtered
form
aqueous solution
potency
0.03-3.0 ng/mL ED50/EC50
quality
endotoxin tested
packaging
pkg of 1 and 5 mL
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... FGF2(2247)
1 of 4
This Item | F5542 | F3685 | F5392 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| biological source human | biological source human | biological source human | biological source bovine pituitary glands |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form aqueous solution | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
General description
Fibroblast growth factor 2 (FGF2), a canonical member, belongs to the fibroblast growth factor family.[1]
Application
Fibroblast Growth Factor-Basic Heparin Stabilized human has been used to culture Brown Norway rat yolk sac epithelial cells transformed with mouse sarcoma virus (BN/MSV).[2] It has also been used in the preparation of serum-free mouse tubal epithelium growth medium (M-TE-GM) to culture primary tubal epithelium (TE) cells.[3]
Biochem/physiol Actions
Fibroblast growth factor 2 (FGF2) participates in tissue development and repair.[1] It is also involved in embryonic development, angiogenesis, bone regeneration, stem cell self-renewal, and wound healing. The basic fibroblast growth factor (bFGF) plays a key role in the progression and maintenance of the nervous system.[4] FGF2 binds to the FGF receptor (FGFR) and activates the extracellular signal-regulated kinase (ERK) pathway, which controls cellular behavior, using heparan sulfates (HS)/heparin as a cofactor.[1] bFGF stimulates cell proliferation, differentiation, and migration in a variety of cell types.[4] In cultured astrocytes from rat brain, recombinant human basic fibroblast growth factor (rhbFGF) may induce nerve growth factor (NGF) secretion.[5] It is the heparin stabilized form of a natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Thi H Nguyen et al.
Nature chemistry, 5(3), 221-227 (2013-02-21)
Basic fibroblast growth factor (bFGF) is a protein that plays a crucial role in diverse cellular functions, from wound healing to bone regeneration. However, a major obstacle to the widespread application of bFGF is its inherent instability during storage and
Zuzana Koledova et al.
Frontiers in cell and developmental biology, 7, 331-331 (2020-01-11)
Fibroblast growth factor 2 (FGF2) plays important roles in tissue development and repair. Using heparan sulfates (HS)/heparin as a cofactor, FGF2 binds to FGF receptor (FGFR) and induces downstream signaling pathways, such as ERK pathway, that regulate cellular behavior. In
Andrea Flesken-Nikitin et al.
Journal of visualized experiments : JoVE, (115) (2016-09-30)
Orthotopic transplantation assays in mice are invaluable for studies of cell regeneration and neoplastic transformation. Common approaches for orthotopic transplantation of ovarian surface and tubal epithelia include intraperitoneal and intrabursal administration of cells. The respective limitations of these methods include
Olivier Cases et al.
The Journal of biological chemistry, 288(23), 16655-16670 (2013-04-18)
Cubilin (Cubn) is a multiligand endocytic receptor critical for the intestinal absorption of vitamin B12 and renal protein reabsorption. During mouse development, Cubn is expressed in both embryonic and extra-embryonic tissues, and Cubn gene inactivation results in early embryo lethality
H Fukumoto et al.
Neuroscience letters, 122(2), 221-224 (1991-01-28)
Recombinant human basic fibroblast growth factor (rhbFGF) was found to induce secretion of nerve growth factor (NGF) in cultured astrocytes from rat brain. The induction was concentration dependent; a maximum increase of 2-fold was observed at concentrations of 3-10 ng/ml
Articles
Fibroblast growth factors in cell culture and various growth factors for your research
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