F9890

Sigma-Aldrich

Monoclonal Anti-Human IgG4−FITC antibody produced in mouse

clone HP-6025, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-Human IgG4
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP-6025, monoclonal

form

buffered aqueous solution

storage condition

protect from light

application(s)

dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4

isotype

IgG1

conjugate

FITC conjugate

shipped in

dry ice

storage temp.

−20°C

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General description

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.

Specificity

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Application

The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Monoclonal Anti-Human IgG4-FITC antibody produced in mouse has been used in immunohistofluorescence and immunofluorescence assay.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.

Physical form

Solution in 0.01 M phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

An immunofluorescence test for phospholipase-A2-receptor antibodies and its clinical usefulness in patients with membranous glomerulonephritis.
Hoxha E, et al.
Nephrology, Dialysis, and Transplantation, 26(8), 2526-2532 (2011)
Franziska von Haxthausen et al.
Frontiers in immunology, 9, 3035-3035 (2019-01-09)
Membranous nephropathy (MN) is an autoimmune disease caused by binding of circulating antibodies to podocyte antigens in the kidney. For decades and still today primary MN has been considered to have an unspecified IgG4-driven autoimmune genesis, while secondary MN has...
Characterization of a subtype of autoimmune encephalitis with anti?contactin-associated protein-like 2 antibodies in the cerebrospinal fluid, prominent limbic symptoms, and seizures.
Joubert B, et al.
JAMA Neurology, 73(9), 1115-1124 (2016)
J D Isaacs et al.
Clinical and experimental immunology, 106(3), 427-433 (1996-12-01)
It is traditionally held that human IgG4 MoAbs should not deplete target cells in vivo, as this isotype is inactive in a number of in vitro assays that measure effector function. We have previously challenged this dogma, and the current...
IgG4-related disease.
Mahajan V S, et al.
Annual Review of Pathology, 9, 315-347 (2014)

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