FD2000S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 2,000,000

Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

Quality Level

biological source

synthetic (organic)

form

powder

mol wt

average mol wt 2,000,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

solubility

H2O: soluble 25 mg/mL

conjugate

FITC conjugate

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Conflicting data on the branch lengths implies that the average branch length is less than three glucose units. However, other methods indicate branches of greater than 50 glucose units exist. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Application

Fluorescein-dextran, 2000 kDa, was used for in vivo imaging of brain microvasculature in active mice using a minature integrated fluorecent microscope. FITC-dextran, 2000 kDa (FD-2000) is used in cardiovascular, microcirculation, perfusion and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. Fluorescein-dextran, 2000 kDa, may be used for in vivo imaging of brain microvasculature in active mice using a miniature integrated fluorescent microscope. FITC-dextran has been used to study plant cell wall porosity.
FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran.
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Packaging

1, 5 g in poly bottle
100, 250 mg in poly bottle

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Denian Wang et al.
Scientific reports, 7(1), 2365-2365 (2017-05-26)
Although anti-angiogenic (AA) therapy is widely used in clinical practice, it is often challenged by insufficient efficacy and intrinsic resistance. Some studies have reported that AA therapy can even increase tumor metastasis. However, whether this is due to a specific...
Weiguo Chen et al.
American journal of respiratory cell and molecular biology, 50(2), 328-336 (2013-09-14)
The statins are now recognized to have pleiotropic properties, including augmentation of endothelial barrier function. To explore the mechanisms involved, we investigated the effect of simvastatin on endothelial cell (EC) tight junctions. Western blotting of human pulmonary artery ECs treated...
Baoqiang Li et al.
PloS one, 14(8), e0221547-e0221547 (2019-08-31)
Chronic atherosclerosis may cause cerebral hypoperfusion and inadequate brain oxygenation, contributing to the progression of cognitive decline. In this study, we exploited two-photon phosphorescence lifetime microscopy to measure the absolute partial pressure of oxygen (PO2) in cortical tissue in both...
Haruna Hirata et al.
Biological & pharmaceutical bulletin, 37(2), 262-267 (2014-02-05)
Long-term peritoneal dialysis (PD) frequently produces morphological and functional changes of the peritoneum, which makes continuation of PD difficult. Moreover, the progression of peritoneal injury causes complications and poor prognosis. Since therapeutic treatments for peritoneal injury during PD have yet...
Poornima Venkat et al.
Neurobiology of aging, 50, 96-106 (2016-12-13)
We investigated cognitive function, axonal/white matter (WM) changes and glymphatic function of vascular dementia using a multiple microinfarction (MMI) model in retired breeder (RB) rats. The MMI model induces significant (p < 0.05) cognitive decline that worsens with age starting...
Related Content
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.