FD40S

Sigma-Aldrich

Fluorescein isothiocyanate–dextran

average mol wt 40,000

Synonym(s):
FITC–Dextran
CAS Number:
MDL number:
NACRES:
NA.25

Quality Level

biological source

synthetic (organic)

form

powder

mol wt

average mol wt 40,000

extent of labeling

0.003-0.020 mol FITC per mol glucose

solubility

H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange

conjugate

FITC conjugate

storage temp.

2-8°C

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General description

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9.

Application

Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:
  • Fluorescein-dextran microscopy.
  • To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.
  • To quantify endocytic activity by flow cytometry.
  • To label α-lactalbumin and ribonuclease.
FITC-dextran is suitable for extensive microcirculation and cell permeability research utilizing microfluorimetry. It is also suitable for the study of plant cell wall porosity and capillary permeability.
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.

Packaging

100, 250 mg in poly bottle
1 g in poly bottle

Linkage

Similar to FD-40, but produced by Sigma.

Preparation Note

Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.

Other Notes

Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Erika M J Siren et al.
Scientific reports, 8(1), 7581-7581 (2018-05-17)
Here we present a simple technique for re-directing reactions on the cell surface to the outermost region of the glycocalyx. Macromolecular crowding with inert polymers was utilized to reversibly alter the accessibility of glycocalyx proteoglycans toward cell-surface reactive probes allowing...
Molecular weight, molecular-weight distribution and molecular size of native dextran.
AROND L H and H P FRANK
The Journal of Physical Chemistry, 953 - 957 (1954)
Yun Mi Lee et al.
Journal of diabetes research, 2013, 245271-245271 (2013-12-29)
Retinal pathogenic angiogenesis in the eyes is a causative factor in retinopathy of prematurity, diabetic retinopathy, and age-related macular degeneration. This study was designed to examine the pathogenic role of the high-mobility group box-1 (HMGB1) protein and the inhibitory effect...
Yun Mi Lee et al.
BMC complementary and alternative medicine, 15, 271-271 (2015-08-13)
Retinal neovascularization is a common cause of vision loss in proliferative diabetic retinopathy, retinopathy of prematurity and age-related macular degeneration. Samul-tang (SMT) is a widely used traditional herbal medicine in East Asia and is also known as Shimotsu-to in Japanese...
The structure of NRRL B-512F dextran, methylation studies.
Van Cleve JW, Schacfer WC and Rist CE
Journal of the American Chemical Society, 4435 - 4438 null
Related Content
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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