FG0465

SAFC

Iscove′s Modified Dulbecco′s Medium

With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture

Quality Level

sterility

sterile-filtered
sterile

product line

AQmedia

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

glucose: yes
NaHCO3: yes
stable glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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