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RPMI-1640 Medium

With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: yes
stable glutamine: yes
sodium pyruvate: no
NaHCO3: yes

shipped in


storage temp.


General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.


RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

M Granados et al.
Journal of cardiovascular translational research, 10(4), 374-390 (2017-05-04)
Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1%
Andres Binolfi et al.
Nature communications, 7, 10251-10251 (2016-01-26)
Cellular oxidative stress serves as a common denominator in many neurodegenerative disorders, including Parkinson's disease. Here we use in-cell NMR spectroscopy to study the fate of the oxidation-damaged Parkinson's disease protein alpha-synuclein (α-Syn) in non-neuronal and neuronal mammalian cells. Specifically
Julia Huetter et al.
Cancer immunology research, 8(7), 895-911 (2020-04-22)
The immunoglobulin-like domain containing receptor 2 (ILDR2), a type I transmembrane protein belonging to the B7 family of immunomodulatory receptors, has been described to induce an immunosuppressive effect on T-cell responses. Besides its expression in several nonlymphoid tissue types, we

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