All Photos(1)

FG1215

Sigma-Aldrich

RPMI-1640 Medium

With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered
sterile

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
stable glutamine: yes
sodium pyruvate: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Application

RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Julia Huetter et al.
Cancer immunology research, 8(7), 895-911 (2020-04-22)
The immunoglobulin-like domain containing receptor 2 (ILDR2), a type I transmembrane protein belonging to the B7 family of immunomodulatory receptors, has been described to induce an immunosuppressive effect on T-cell responses. Besides its expression in several nonlymphoid tissue types, we...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service