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G0154

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

seawater medium

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Pack SizeSKUAvailabilityPrice
500 mL
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$89.80

About This Item

NACRES:
NA.72
UNSPSC Code:
10171502

$89.80

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sterility

sterile-filtered

Quality Level

400

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:
  • for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
  • to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
  • as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Preparation Note

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder
Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

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Guillard′s (F/2) Marine Water Enrichment Solution

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form

liquid

form

liquid

form

liquid

form

liquid

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

-

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL7293
RNBL0701
RNBK0704
RNBJ0050
RNBH4653

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Protocols

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Gilles Defrel et al.
Frontiers in bioengineering and biotechnology, 9, 734902-734902 (2021-10-19)
Efficient and reliable genome engineering technologies have yet to be developed for diatoms. The delivery of DNA in diatoms results in the random integration of multiple copies, quite often leading to heterogeneous gene activity, as well as host instability. Transgenic
Girish Beedessee et al.
Scientific reports, 9(1), 1204-1204 (2019-02-06)
Symbiodiniaceae dinoflagellates possess smaller nuclear genomes than other dinoflagellates and produce structurally specialized, biologically active, secondary metabolites. Till date, little is known about the evolution of secondary metabolism in dinoflagellates as comparative genomic approaches have been hampered by their large
Shao-En Peng et al.
PeerJ, 8, e9745-e9745 (2020-11-17)
The mutualistic symbiosis between anthozoans and intra-gastrodermal dinoflagellates of the family Symbiodiniaceae is the functional basis of all coral reef ecosystems, with the latter providing up to 95% of their fixed photosynthate to their hosts in exchange for nutrients. However
View All Related Papers

Global Trade Item Number

SKUGTIN
G0154-500ML04061833623336

Questions

1–3 of 3 Questions  

  1. The nutrients and vitamins come disoved in ditiled water, or salt-water? I mean, if I add 20 mL to 980 mL of seawater to make the culture medium, am I diluting a litle the marine water?

    1 answer

    1. G0154 is made using our standard WFI-grade water.
      Guillard?s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard?s (F/2) Marine Enrichment medium. We recommend diluting 20mL of G0154 in 1L seawater.
      https://www.sigmaaldrich.com/US/en/product/sigma/g0154

      The composition of G0154 can be found in the link below:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media#table

      Helpful?

  2. how many milliliters of the product should i use in 1 liter of sea water? thank you in advance.

    1 answer

    1. Use 20ml of this product per Liter of seawater.

      Helpful?

  3. Does this come in a liquid or powder form? Because in "form" it is listed as liquid, but under "general description" it reads as "a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater.

    1 answer

    1. This product is a 50X concentrate in liquid form. The 500 mL package size will prepare 25 L of medium at 1X concentration.

      Helpful?

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