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G0154

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

Synonym(s):

seawater medium

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Size/SKUAvailabilityPrice
500 mL
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$93.00

About This Item

NACRES:
NA.72
UNSPSC Code:
10171502

$93.00

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sterility

sterile-filtered

Quality Segment

form

liquid

concentration

50 ×

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:
  • for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
  • to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
  • as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Preparation Note

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder
Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

1–3 of 3 Questions  

  1. The nutrients and vitamins come disoved in ditiled water, or salt-water? I mean, if I add 20 mL to 980 mL of seawater to make the culture medium, am I diluting a litle the marine water?

    1 answer

    1. G0154 is made using our standard WFI-grade water.
      Guillard?s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard?s (F/2) Marine Enrichment medium. We recommend diluting 20mL of G0154 in 1L seawater.
      https://www.sigmaaldrich.com/US/en/product/sigma/g0154

      The composition of G0154 can be found in the link below:
      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/plant-tissue-culture/phycology-media#table

      Helpful?

  2. how many milliliters of the product should i use in 1 liter of sea water? thank you in advance.

    1 answer

    1. Use 20ml of this product per Liter of seawater.

      Helpful?

  3. Does this come in a liquid or powder form? Because in "form" it is listed as liquid, but under "general description" it reads as "a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater.

    1 answer

    1. This product is a 50X concentrate in liquid form. The 500 mL package size will prepare 25 L of medium at 1X concentration.

      Helpful?

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