G0154

Guillard′s (F/2) Marine Water Enrichment Solution

50 ×, liquid, suitable for plant cell culture

• Synonym(s): seawater medium
• NACRES: NA.72

Properties

• sterility: sterile-filtered
• Quality Level: 400
• form: liquid
• concentration: 50 ×
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Guillard′s (F/2) Marine Water Enrichment Solution 50 X is a concentrate of major nutrients, trace metals, and vitamins that can be diluted in filtered seawater to support the reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing coastal marine algae (diatoms).

Application

Guillard′s (F/2) Marine Water Enrichment Solution has been used:

• for culturing Chromera velia CCMP2878 to study coral response to Chromera infection
• to enrich Baltic Sea water to culture unialgal stock of Symbiodinium strains
• as a supplement in water culture medium to culture Chaetoceros affinis (CA) inoculum

Guillard′s (F/2) Marine Water Enrichment Solution 50 X, is a concentrate of major nutrients, trace metals and vitamins that can be diluted in filtered seawater to support reconstitution of Guillard′s (F/2) Marine Enrichment medium. Guillard′s (F/2) Marine Enrichment medium is a widely used enriched seawater medium for growing costal marine algae (diatoms).

Formula variant

Without silicate. With the major nutrients, trace metals and vitamins as described by Guillard (1975).

Reconstitution

Use at 20ml/L of seawater.

Phycology Media composition and preparation from powder

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable