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G0411

Sigma-Aldrich

Prionex® Highly purified Type A

aqueous solution

Synonym(s):
Gelatin, Prionex® Highly purified Type A
CAS Number:
EC Number:
MDL number:

Quality Level

biological source

Porcine bone

sterility

aseptically processed

form

aqueous solution

application(s)

ELISA: suitable
microbiological culture: suitable
western blot: suitable

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue. Gelatin is a biocompatible polymer.

Application

Prionex® Highly purified Type A has been used:
  • for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA
  • as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry
  • as a component of media for species differentiation in bacteriology
  • as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for engineering applications
  • in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo

Legal Information

Prionex is a registered trademark of Pentapharm AG, Basel

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Photographic Processes and Materials
Encyclopedia of Physical Science and Technology, 91-132 (2001)
Food Gels
Encyclopedia of Materials: Science and Technology, 3231-3233 (2001)
Gels for Photographic Emulsions
Encyclopedia of Materials: Science and Technology, 3495-3497 (2001)
PCR Kit
PCR Primer: A Laboratory Manual (1995)
Amino Acid Composition of Gelatin from Ephinephelus sp.
Siregar GRM and Suprayitno E
IOSR Journal of Agriculture and veterinary Science, 12(4), 51-54 (2019)

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