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G0411

Sigma-Aldrich

Prionex® Highly purified Type A

aqueous solution

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Synonym(s):
Gelatin, Prionex® Highly purified Type A
CAS Number:
EC Number:
MDL number:

biological source

Porcine bone

Quality Level

sterility

aseptically processed

form

aqueous solution

technique(s)

ELISA: suitable
microbiological culture: suitable
western blot: suitable

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G1393G1890G9391
Sigma-Aldrich

G0411

Prionex® Highly purified Type A

Sigma-Aldrich

G1393

Gelatin solution

Gelatin from porcine skin powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

G1890

Gelatin from porcine skin

Gelatin from bovine skin Type B, powder, BioReagent, suitable for cell culture

G9391

Gelatin from bovine skin

technique(s)

ELISA: suitable, western blot: suitable, microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

form

aqueous solution

form

solution

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

sterility

aseptically processed

sterility

sterile

sterility

sterile

sterility

sterile

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue. Gelatin is a biocompatible polymer.

Application

Prionex® Highly purified Type A has been used:
  • for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA
  • as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry
  • as a component of media for species differentiation in bacteriology
  • as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for engineering applications
  • in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo

Legal Information

Prionex is a registered trademark of Pentapharm AG, Basel

Storage Class

11 - Combustible Solids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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