β-Glucuronidase from Helix pomatia can be stored at –20 °C. There is no expiration date or recommended retest date on the Certificate of Analysis, however the data sheet states when stored at –20 °C, states this enzyme retains activity for at least 3 years.
G0751
β-Glucuronidase from Helix pomatia
Type H-1, partially purified powder, ≥300,000 units/g solid
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100000 units | Available to ship TODAYfromMILWAUKEE | $97.50 | |
| 500000 units | Available to ship TODAYfromMILWAUKEE | $310.00 | |
| 1000000 units | Available to ship TODAYfromMILWAUKEE | $432.00 | |
| 2000000 units | Available to ship TODAYfromMILWAUKEE | $700.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥300,000 units/g solid
$97.50
Available to ship TODAYDetails
type
Type H-1
Quality Level
form
partially purified powder
specific activity
≥300,000 units/g solid
secondary activity
≥10,000 units/g solid sulfatase
storage temp.
−20°C
General description
β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥300,000 units/g solid | specific activity ≥400,000 units/g solid | specific activity ≥90,000 units/mL | specific activity ≥90,000 units/mL |
| form partially purified powder | form lyophilized powder | form aqueous solution | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| type Type H-1 | type Type H-5 | type Type H-3 | type Type HP-2S |
| secondary activity ≥10,000 units/g solid sulfatase | secondary activity ≤40,000 units/g solid sulfatase | secondary activity ≤1,000 units/mL sulfatase | secondary activity ≤7,500 units/mL sulfatase |
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signalword
Danger
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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Protocols
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| G0751-500KU | 04061835556953 |
| G0751-100KU | 04061833624142 |
| G0751-2MU | 04061833624166 |
| G0751-1MU | 04061833624159 |
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Does this have an expiration date? Specifically for lot SLCL6750?
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What is the weight per unit of this product?
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The specification of this product is 300,000 units/g solid. The specific unit value will be determined for each lot of product and reported on the lot-specific CofA document.
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