G0762

β-Glucuronidase from Helix pomatia

Name: β-Glucuronidase from <I>Helix pomatia</I>
Brand: SIGMA
Price: 84.5 USD

Type H-3AF, aqueous solution, ≥60,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

Slide 1 of 3

Select a Size

100000 UNITS

$84.50

Check Cart for Availability

About This Item

CAS Number:

9001-45-0

EC Number:

3.2.1.31 (BRENDA, IUBMB)

MDL number:

MFCD00130629

UNSPSC Code:

12352204

NACRES:

NA.54

Key Documents

View All Documentation

biological source

Helix pomatia

type

Type H-3AF

form

aqueous solution

specific activity

≥60,000 units/mL

impurities

≤1,000 unit/mL Sulfatase

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	G0876	G0751	G1512
Sigma-Aldrich G0762 β-Glucuronidase from Helix pomatia	Sigma-Aldrich G0876 β-Glucuronidase from Helix pomatia	Sigma-Aldrich G0751 β-Glucuronidase from Helix pomatia	Sigma-Aldrich G1512 β-Glucuronidase from Helix pomatia
specific activity ≥60,000 units/mL	specific activity ≥85,000 units/mL	specific activity ≥300,000 units/g solid	specific activity ≥400,000 units/g solid
biological source Helix pomatia	biological source -	biological source -	biological source -
form aqueous solution	form aqueous solution	form partially purified powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in wet ice	shipped in -	shipped in -
Quality Level 300	Quality Level 300	Quality Level 200	Quality Level 200

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

A further processing of Type H-3 to remove agglutinin.

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

used together

Product No.

Description

Pricing

B8174

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, tablet

B4532

5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98%

73677

4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

0000497759

0000464749

SLCM7782

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Correlation of oestriol levels in saliva, plasma and urine of pregnant women.

W Lechner et al.

Acta endocrinologica, 109(2), 266-268 (1985-06-01)

Unconjugated and total oestriol (E3) have been determined in saliva (S) and plasma (P) by radioimmunoassay using a highly specific antiserum against E3. Total oestrogens (E = E1 + E2 + E3) in urine were analysed by the Kober-Ittrich method.

Reduced Klotho is associated with the presence and severity of coronary artery disease.

Juan F Navarro-González et al.

Heart (British Cardiac Society), 100(1), 34-40 (2013-10-30)

Klotho is involved in vascular health. We aimed to analyse in a cross-sectional study the relationship between Klotho and human coronary artery disease (CAD). The study included 371 subjects who underwent coronary angiography and 70 patients who underwent elective cardiac

Neutrophils contribute to inflammatory lymphangiogenesis by increasing VEGF-A bioavailability and secreting VEGF-D.

Kar Wai Tan et al.

Blood, 122(22), 3666-3677 (2013-10-12)

Lymphangiogenesis is an important physiological response to inflammatory insult, acting to limit inflammation. Macrophages, dendritic cells, and lymphocytes are known to drive lymphangiogenesis. In this study, we show that neutrophils recruited to sites of inflammation can also coordinate lymphangiogenesis. In

Regulation of renal phosphate transport by FGF23 is mediated by FGFR1 and FGFR4.

Jyothsna Gattineni et al.

American journal of physiology. Renal physiology, 306(3), F351-F358 (2013-11-22)

Fibroblast growth factor 23 (FGF23) is a bone-derived hormone that acts on the proximal tubule to decrease phosphate reabsorption and serum levels of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂ Vitamin D₃]. Abnormal FGF23 metabolism has been implicated in several debilitating hypophosphatemic and hyperphosphatemic

βKlotho suppresses tumor growth in hepatocellular carcinoma by regulating Akt/GSK-3β/cyclin D1 signaling pathway.

Xiaoming Ye et al.

PloS one, 8(1), e55615-e55615 (2013-02-06)

βKlotho is a regulator in multiple metabolic processes, while its role in cancer remains unclear. We found the expression of βKlotho was down-regulated in human hepatocellular carcinoma tissues compared with that in paired adjacent non-tumourous liver tissues. Hepatoma cells also

View All Related Papers

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service