G0792

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor from rat

GM-CSF, recombinant, expressed in E. coli

Synonym(s):
GM-CSF
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

rat

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.1 ng/mL

quality

endotoxin tested

mol wt

14.7 kDa

packaging

pkg of 5X5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... CSF2(116630), Csf2(116630)

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Biochem/physiol Actions

Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Preparation Note

Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

Analysis Note

The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Julie M Vose et al.
Leukemia & lymphoma, 50(9), 1412-1421 (2009-07-16)
In autologous stem cell transplantation, mobilized peripheral blood has replaced the bone marrow as the preferred source of hematopoietic stem cells (HSCs). Because HSCs normally exist in the blood in very low numbers, the use of agents to "mobilize" HSCs...
Stephanie S Watowich et al.
Immunological reviews, 238(1), 76-92 (2010-10-26)
Understanding the diversification of dendritic cell (DC) lineages is one of the last frontiers in mapping the developmental hierarchy of the hematopoietic system. DCs are a vital link between the innate and adaptive immune responses; thus, elucidating their developmental pathways...
Fengjiao Yuan et al.
PloS one, 9(9), e107063-e107063 (2014-09-10)
Cigarette smoking is a major pathogenic factor in lung cancer. Macrophages play an important role in host defense and adaptive immunity. These cells display diverse phenotypes for performing different functions. M2 type macrophages usually exhibit immunosuppressive and tumor-promoting characteristics. Although...
Antonella Mancusi et al.
Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in...
Mingming Zhang et al.
PloS one, 9(10), e109146-e109146 (2014-10-03)
It has been shown that Faecalibacterium prausnitzii (F. prausnitzii), one of the dominant intestinal bacterial flora, may protect colonic mucosa against the development of inflammation and subsequent inflammatory bowel disease (IBD), with the underlying mechanisms being unclear. The impacts of...

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