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G0876

β-Glucuronidase from Helix pomatia

Type H-2, aqueous solution, ≥85,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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$264.00

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About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629

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Product Name

β-Glucuronidase from Helix pomatia, Type H-2, aqueous solution, ≥85,000 units/mL

type

Type H-2

form

aqueous solution

specific activity

≥85,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

shipped in

wet ice

storage temp.

2-8°C

Quality Level

300

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This Item
G8885G0762G7017
β-Glucuronidase from Helix pomatia Type H-2, aqueous solution, ≥85,000 units/mL

Sigma-Aldrich

G0876

β-Glucuronidase from Helix pomatia

β-Glucuronidase from Helix pomatia Type H-3, aqueous solution, ≥90,000 units/mL

Sigma-Aldrich

G8885

β-Glucuronidase from Helix pomatia

β-Glucuronidase from Helix pomatia Type H-3AF, aqueous solution, ≥60,000 units/mL

Sigma-Aldrich

G0762

β-Glucuronidase from Helix pomatia

β-Glucuronidase from Helix pomatia Type HP-2, aqueous solution, ≥100,000 units/mL

Sigma-Aldrich

G7017

β-Glucuronidase from Helix pomatia

specific activity

≥85,000 units/mL

specific activity

≥90,000 units/mL

specific activity

≥60,000 units/mL

specific activity

≥100,000 units/mL

form

aqueous solution

form

aqueous solution

form

aqueous solution

form

aqueous solution

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

300

type

Type H-2

type

Type H-3

type

Type H-3AF

type

Type HP-2

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Physical form

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000502286
0000502285
0000502286
0000502285
0000500556

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Aynun Nahar Begum et al.
The Journal of nutrition, 134(1), 120-127 (2004-01-06)
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Han-Hsing Tsou et al.
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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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