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G1272

Sigma-Aldrich

Gentamicin solution

10 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

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Synonym(s):
Garamycin, Gentiomycin C
CAS Number:
EC Number:
MDL number:
eCl@ss:
42020823
PubChem Substance ID:
NACRES:
NA.76

biological source

microbial

Quality Level

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

potency

10-12 mg per mL

concentration

10 mg/mL in deionized water

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

colorless to light yellow

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycoplasma

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.CN[C@@H]1[C@@H](O)[C@H](OC[C@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H](CN)CC[C@H]3N)[C@@H]2O.CN[C@@H]4[C@@H](O)[C@H](OC[C@]4(C)O)O[C@H]5[C@H](N)C[C@H](N)[C@@H](O[C@H]6O[C@@H](CC[C@H]6N)[C@@H](C)N)[C@@H]5O.CN[C@H](C)[C@@H]7CC[C@@H](N)[C@H](O7)O[C@@H]8[C@@H](N)C[C@@H](N)[C@H](O[C@H]9OC[C@](C)(O)[C@H](NC)[C@H]9O)[C@H]8O

InChI

1S/C21H43N5O7.C20H41N5O7.C19H39N5O7.H2O4S/c1-9(25-3)13-6-5-10(22)19(31-13)32-16-11(23)7-12(24)17(14(16)27)33-20-15(28)18(26-4)21(2,29)8-30-20;1-8(21)12-5-4-9(22)18(30-12)31-15-10(23)6-11(24)16(13(15)26)32-19-14(27)17(25-3)20(2,28)7-29-19;1-19(27)7-28-18(13(26)16(19)24-2)31-15-11(23)5-10(22)14(12(15)25)30-17-9(21)4-3-8(6-20)29-17;1-5(2,3)4/h9-20,25-29H,5-8,22-24H2,1-4H3;8-19,25-28H,4-7,21-24H2,1-3H3;8-18,24-27H,3-7,20-23H2,1-2H3;(H2,1,2,3,4)/t9-,10-,11+,12-,13+,14+,15-,16-,17+,18-,19-,20-,21+;8-,9-,10+,11-,12+,13+,14-,15-,16+,17-,18-,19-,20+;8-,9+,10-,11+,12-,13+,14+,15-,16+,17+,18+,19-;/m110./s1

InChI key

RDEIXVOBVLKYNT-HDZPSJEVSA-N

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This Item
G1397G3632G1914
Gentamicin solution 10 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

G1272

Gentamicin solution

Gentamicin solution 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

G1397

Gentamicin solution

Gentamicin sulfate meets USP testing specifications, powder

Sigma-Aldrich

G1914

Gentamicin sulfate

sterility

0.1 μm filtered

sterility

0.1 μm filtered

sterility

-

sterility

-

product line

BioReagent

product line

BioReagent

product line

-

product line

-

form

liquid

form

liquid

form

powder

form

powder

potency

10-12 mg per mL

potency

50-60 mg per mL

potency

≥590 I.U. Gentamicin base per mg, ≥590 units/mg Gentamicin

potency

-

concentration

10 mg/mL in deionized water

concentration

50 mg/mL in deionized water

concentration

50 μg/mL in H2O (is the recommended working concentration for eukaryotic cell culture)

concentration

-

General description

Chemical structure: aminoglycoside

Application

Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 10 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter.

Biochem/physiol Actions

Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.

Components

Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.

Caution

The product is stable at 37° for 5 days and is stored at 2-8°C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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