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G1279

Sigma-Aldrich

G 418 disulfate salt

suitable for plant cell culture, BioReagent

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Synonym(s):
Antibiotic G418
Empirical Formula (Hill Notation):
C20H40N4O10 · 2H2SO4
CAS Number:
Molecular Weight:
692.71
MDL number:
PubChem Substance ID:
NACRES:
NA.72

product line

BioReagent

form

powder

potency

≥720 μg per mg (Dried basis)

technique(s)

cell culture | plant: suitable

color

white to off-white

solubility

100 mg/mL (H2O clear)

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI key

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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This Item
G5013A1720G8168
G 418 disulfate salt suitable for plant cell culture, BioReagent

Sigma-Aldrich

G1279

G 418 disulfate salt

G 418 disulfate salt potency: ≥720 μg per mg (dried basis)

Sigma-Aldrich

G5013

G 418 disulfate salt

G 418 disulfate salt powder, BioReagent, suitable for cell culture

Sigma-Aldrich

A1720

G 418 disulfate salt

form

powder

form

powder

form

powder

form

solution

potency

≥720 μg per mg (Dried basis)

potency

≥720 μg per mg (dried basis)

potency

≥720 μg per mg (Dried basis)

potency

50-60 mg per mL

technique(s)

cell culture | plant: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

color

white to off-white

color

white to off-white

color

white to off-white

color

colorless

solubility

100 mg/mL (H2O clear)

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)

solubility

H2O: 50 mg/mL, clear

General description

Chemical structure: aminoglycoside

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Caution

The product is stable for three years when stored at 2-8°C.

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Luis M Molinos-Albert et al.
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The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and
Elsa A Marquez et al.
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Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

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