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G1393

Sigma-Aldrich

Gelatin solution

Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture

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CAS Number:
MDL number:
NACRES:
NA.75

biological source

bovine hide

Quality Level

sterility

sterile

type

Type B

product line

BioReagent

form

solution

packaging

pkg of 100 mL
pkg of 20 mL

concentration

2% in H2O, tissue culture grade

technique(s)

cell culture | mammalian: suitable

surface coverage

100‑200 μg/cm2

impurities

endotoxin, tested

solubility

water: soluble

Binding Specificity

Peptide Source: Fibrinogen

shipped in

ambient

storage temp.

2-8°C

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This Item
G9391C4243C2124
Sigma-Aldrich

Sigma-Aldrich

G1393

Gelatin solution

sterility

sterile

sterility

sterile

sterility

sterile-filtered

sterility

sterile-filtered

form

solution

form

powder

form

liquid

form

liquid

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 100 g, pkg of 500 g

packaging

pkg of 100 mL, pkg of 20 mL

packaging

pkg of 50 mL

concentration

2% in H2O, tissue culture grade

concentration

-

concentration

2.9-3.2 mg/mL

concentration

6 mg/mL

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue. This is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry. It takes up random coil structure after digestion from the triple helical collagen. The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objective.

Application

Gelatin solution has been used:
  • for coating of cell culture plates or dishes used for embryonic stem cell culture, testicular cell culture and neural rosettes
  • in coating cell culture to improve attachment of cells
  • in PCR to stabilize Taq DNA
  • as a blocking reagent in western blotting
  • in ELISA
  • in immunochemistry
  • as a component of media for species differentiation in bacteriology
  • to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes
  • to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo

Biochem/physiol Actions

Gelatin has gelling property and displays surface behaviour for use in foams and adhesions. It also serves as a clarification agent in fruit based juices. It is a digestible protein and serves as a binding agent, thickener, stabilizer and emulsifier in food industry. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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