G1401

Sigma-Aldrich

Glial Cell Line-derived Neurotrophic Factor from rat

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Synonym(s):
ATF, GDNF, Astrocyte-derived trophic factor
MDL number:
NACRES:
NA.32

Quality Level

biological source

rat

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.10-10 ng/mL

mol wt

~30 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... Gdnf(25453)

Biochem/physiol Actions

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid (TFA) containing 50 μg bovine serum albumin (BSA) per 1 μg of cytokine.

Analysis Note

The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Roche C de Guzman et al.
Journal of microencapsulation, 25(7), 487-498 (2009-02-25)
Controlled expression of glial cell line derived neurotrophic factor (Gdnf) can be integrated in the development of a system for repair of injured peripheral nerves. This delivery strategy was demonstrated via inducible Gdnf from microencapsulated cells in barium alginate. The...
Weidong Xiao et al.
Molecular neurobiology, 50(2), 274-289 (2014-06-01)
Acute intestinal ischemia reperfusion (IR) injury is often associated with intestinal epithelial barrier (IEB) dysfunction. Enteric glial cells (EGCs) play an essential role in maintaining the integrity of IEB functions. However, the precise mechanism of EGCs under IR stimulation remains...
Clive Bate et al.
Molecular neurodegeneration, 3, 1-1 (2008-01-09)
The early stages of Alzheimer's disease (AD) are closely associated with the production of the Abeta1-42 peptide, loss of synapses and gradual cognitive decline. Since some epidemiological studies showed that EGb 761, an extract from the leaves of the Ginkgo...
Michela Morano et al.
International journal of nanomedicine, 9, 5289-5306 (2014-12-09)
Innovative nerve conduits for peripheral nerve reconstruction are needed in order to specifically support peripheral nerve regeneration (PNR) whenever nerve autotransplantation is not an option. Specific support of PNR could be achieved by neurotrophic factor delivery within the nerve conduits...
Gunnar H D Poplawski et al.
Nature, 581(7806), 77-82 (2020-05-08)
Grafts of spinal-cord-derived neural progenitor cells (NPCs) enable the robust regeneration of corticospinal axons and restore forelimb function after spinal cord injury1; however, the molecular mechanisms that underlie this regeneration are unknown. Here we perform translational profiling specifically of corticospinal...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.