This product has not been validated for immunohistochemistry (IHC). However, numerous citations exist using this product for IHC.
G1544
Anti-GFP, N-terminal antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Green Fluorescent Protein
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100 μG
$551.00
$551.00
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Product Name
Anti-GFP, N-terminal antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates
western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
1 of 4
This Item | G1546 | G6539 | D1067 |
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form affinity isolated antibody |
| biological source rabbit | biological source mouse | biological source mouse | biological source rabbit |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| technique(s) immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts | technique(s) indirect ELISA: suitable, western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins | technique(s) dot blot: suitable, indirect ELISA: suitable, western blot: 1:2,000 using purified recombinant GFP preparation | technique(s) immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR, western blot (chemiluminescent): 0.5-1.0 μg/mL using 100 ng of purified recombinant DHFR |
Application
Anti-GFP, N-terminal antibody produced in rabbit has been used:
- in immunoblotting
- in the histochemistry and immunohistochemistry
- in the immunoprecipitation
Biochem/physiol Actions
Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.
General description
Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.
Immunogen
synthetic peptide corresponding amino acids 3-17 of GFP.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
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A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells
Gable T, et al.
Cancer letters, 396(6), 85-93 (2017)
Green fluorescent protein (GFP): applications, structure, and related photophysical behavior
Zimmer M
Chemical Reviews, 102(3), 759-782 (2002)
Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros
Liu Y, et al.
Development, 134(6), 1111-1122 (2007)
Pathogenic mechanism of recurrent mutations of SCN8A in epileptic encephalopathy
Wagnon JL, et al.
Annals of Clinical and Translational Neurology, 3(2), 114-123 (2016)
Green fluorescent protein: a perspective
Remington SJ
Protein Science, 20(9), 1509-1519 (2011)
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