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General description
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.
Application
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological media. Gellan gum is used in a wide variety of immobilization matricies.
Gelzan™ CM has been used:
- in the synthesis of gellan-pullulan nanogel via chemical crosslinking
- in the synthesis of gellan-pullulan hydrogel
- with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging
Gelzan™ CM has been used:
- in the synthesis of gellan-pullulan nanogel via chemical crosslinking
- in the synthesis of gellan-pullulan hydrogel
- with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging
Features and Benefits
- Transparent and colorless agar alternative
- Mechanically robust
- Disperses in water with ease
- Low viscosity
- The resulting gel has a high melting temperature
Legal Information
Gelrite is a registered trademark of CP Kelco U.S., Inc.
Gelzan is a trademark of CP Kelco U.S., Inc.
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Timothy E L Douglas et al.
Journal of biomedical materials research. Part A, 106(3), 822-828 (2017-10-24)
Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles
Timothy E L Douglas et al.
Biomedical materials (Bristol, England), 12(2), 025015-025015 (2017-02-23)
Injectable composites for tissue regeneration can be developed by dispersion of inorganic microparticles and cells in a hydrogel phase. In this study, multifunctional carbonate microparticles containing different amounts of calcium, magnesium and zinc were mixed with solutions of gellan gum
Marco A Lopez-Heredia et al.
Journal of tissue engineering and regenerative medicine, 12(8), 1825-1834 (2018-04-28)
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.
Gustavo de Medeiros et al.
Nature communications, 6, 8881-8881 (2015-11-26)
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light
J T Oliveira et al.
Journal of tissue engineering and regenerative medicine, 3(7), 493-500 (2009-07-15)
Gellan gum is a polysaccharide that has been recently proposed by our group for cartilage tissue-engineering applications. It is commonly used in the food and pharmaceutical industry and has the ability to form stable gels without the use of harsh
Protocols
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
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