Skip to Content
MilliporeSigma

G1N10

GenElute™ Mammalian Genomic DNA Miniprep Kits

sufficient for 10 purifications

Synonym(s):

Mammalian Genomic DNA Miniprep, Gen Elute

Slide 1 of 5
Sign In to View Organizational & Contract Pricing

Select a Size

1 KIT

$54.30

$54.30

Available to ship TODAYDetails

About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

sufficient for 10 purifications

storage temp.

15-25°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
G1N350G1N70NA2100
GenElute™ Mammalian Genomic DNA Miniprep Kits sufficient for 10 purifications

Sigma-Aldrich

G1N10

GenElute™ Mammalian Genomic DNA Miniprep Kits

GenElute™ Mammalian Genomic DNA Miniprep Kits sufficient for 350 purifications

Sigma-Aldrich

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits

GenElute™ Mammalian Genomic DNA Miniprep Kits sufficient for 70 purifications

Sigma-Aldrich

G1N70

GenElute™ Mammalian Genomic DNA Miniprep Kits

GenElute™ Bacterial Genomic DNA Kits sufficient for 10 purifications

Sigma-Aldrich

NA2100

GenElute™ Bacterial Genomic DNA Kits

usage

sufficient for 10 purifications

usage

sufficient for 350 purifications

usage

sufficient for 70 purifications

usage

sufficient for 10 purifications

G1N350

G1N10, G1N70, G1N9602, G1N9604

G1N10, G1N350, G1N9602, G1N9604

NA2110, NA2120

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient method to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Biochem/physiol Actions

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • R6148RNase A solutionSDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg proteinSDS
  • C2112Column Preparation SolutionSDS

related product

Product No.
Description
Pricing

A9434

Ammonium chloride, Molecular Biology, suitable for cell culture, ≥99.5%

P2308

Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

E1510

Ethidium bromide solution, BioReagent, Molecular Biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, Molecular Biology, low EEO

W4502

Water, Nuclease-Free Water, for Molecular Biology

G1N70

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 70 purifications

D9281

Lambda DNA EcoR I Hind III Digest, for DNA electrophoresis

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

G1N350

GenElute™ Mammalian Genomic DNA Miniprep Kits, sufficient for 350 purifications

R6148

RNase A solution

459836

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

SRE0005

Proteinase K from Tritirachium album, Reagents designed and manufactured under current ISO 13485 certification.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

483.8 °F

flash_point_c

251 °C

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000437403
0000437403
0000474587
0000474587
SLCL7191

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Züleyha Doğanyiğit et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 20(7), 632-639 (2013-03-05)
In recent years, propolis has been the object of extensive research for its antibacterial, antioxidant, anti-inflammatory, and antitumoral activities. This study aims to determine the hepatoprotective efficiency of propolis on experimental endotoxemia in rats. In the current study, fifty adult
Matthew J Coussens et al.
Journal of visualized experiments : JoVE, (62)(62), e3303-e3303 (2012-04-18)
The Target ID Library is designed to assist in discovery and identification of microRNA (miRNA) targets. The Target ID Library is a plasmid-based, genome-wide cDNA library cloned into the 3'UTR downstream from the dual-selection fusion protein, thymidine kinase-zeocin (TKzeo). The
Yuma Yamada et al.
Biomaterials, 33(15), 3952-3958 (2012-03-06)
A quantitative comparison between nuclear DNA release from carriers and their transfection activity would be highly useful for improving the effectiveness of non-viral gene vectors. We previously reported that, for condensed DNA particles, a close relationship exists between the efficiency
Natalia N Pouchkina-Stantcheva et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 138(4), 371-376 (2004-08-25)
Various spider species produce dragline silks with different mechanical properties. The primary structure of silk proteins is thought to contribute to the elasticity and strength of the fibres. Previously published work has demonstrated that the dragline silk of Euprosthenops sp.
Soledad Betanzos-Lara et al.
Dalton transactions (Cambridge, England : 2003), 44(8), 3673-3685 (2015-01-07)
DNA interactions of anticancer mononuclear Cu(2+), Co(2+), Zn(2+), and Ni(2+) complexes with the biologically active ligand clotrimazole (clotri) are reported. To fully characterize DNA binding modes for these complexes of the formulae [M(clotri)2Cl2]·nH2O (1-4), [M(clotri)2Br2]·nH2O (5,6), [M(clotri)3NO3]NO3·nH2O (9), and [M(clotri)3(NO3)2]
View All Related Papers

Articles

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Protocols

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.

FFPE Tissue

Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

See All

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service