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G211250

Sigma-Aldrich

DMT-2′Fluoro-dG(ib) Phosphoramidite

configured for (ÄKTA® and OligoPilot®)

Synonym(s):
DMT-2′Fluoro-dG(ib) Amidite
Empirical Formula (Hill Notation):
C44H53FN7O8P
CAS Number:
Molecular Weight:
857.91
MDL number:
PubChem Substance ID:
NACRES:
NA.21

biological source

non-animal source (no BSE/TSE risk)

Quality Level

product line

Proligo Reagents

assay

≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)
conforms (1H-NMR)
conforms (LC/MS)

form

powder

technique(s)

oligo synthesis: suitable

impurities

≤0.3% water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (reversed phase HPLC)
≤3% residual Solvent content

color

white to off-white

λ

conforms (UV/VIS Identity)

compatibility

configured for (ÄKTA® and OligoPilot®)

nucleoside profile

base: deoxyguanosine
base protecting group: isobutyryl
2' protecting group: fluoro
5' protecting group: DMT
deprotection: fast/standard

storage temp.

−20°C

SMILES string

COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(NC(=O)C(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6

InChI

1S/C44H53FN7O8P/c1-27(2)40(53)49-43-48-39-37(41(54)50-43)47-26-51(39)42-36(45)38(60-61(58-24-12-23-46)52(28(3)4)29(5)6)35(59-42)25-57-44(30-13-10-9-11-14-30,31-15-19-33(55-7)20-16-31)32-17-21-34(56-8)22-18-32/h9-11,13-22,26-29,35-36,38,42H,12,24-25H2,1-8H3,(H2,48,49,50,53,54)/t35-,36-,38-,42-,61?/m1/s1

InChI key

KJFUMXZZVYMQEU-AOCJBPQJSA-N

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General description

DMT-2′Fluoro-dG(ib) Phosphoramidite is a 2′Fluoro phosphoramidite used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance. Its key features are-
  • Can be employed together with DNA or RNA phosphoramidites
  • Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
  • Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
  • Consistent lot-to-lot high purity and performance
  • Manufactured under a certified ISO 9001 quality system

Application

DMT-2′Fluoro-dG(ib) Phosphoramidite is suitable for use in the synthesis of 2′-fluoro modified oligonucleotide sequences by employing automated solid-phase chemistry.

Legal Information

OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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