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G2267

Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

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Synonym(s):
D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

rabbit muscle

Quality Level

form

lyophilized powder

specific activity

≥75 units/mg protein

composition

Protein, ≥80% biuret

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%
lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%
triosephosphate isomerase ≤0.1%

shipped in

wet ice

storage temp.

−20°C

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This Item
G6880P3397P6635
Phosphorylase b from rabbit muscle lyophilized powder, ≥20 units/mg protein, 2× crystallization

P6635

Phosphorylase b from rabbit muscle

biological source

rabbit muscle

biological source

-

biological source

rabbit muscle

biological source

rabbit muscle

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

foreign activity

3-Phosphoglyceric phosphokinase ≤0.05%, triosephosphate isomerase ≤0.1%, lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%

foreign activity

lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%

foreign activity

phosphoglucomutase ≤1.0%, phosphorylase a ≤10%, phosphorylase kinase ≤0.5%, phosphorylase phosphatase, debrancher enzyme, AMPase and ATPase ≤0.1%

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
  • for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
  • to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
  • in anti-aggregation assays
  • in purified GAPDH studies

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing citrate buffer salts

inhibitor

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Poly ADP-Ribose Polymerase Inhibition Ameliorates Hind Limb Ischemia Reperfusion Injury in a Murine Model of Type 2 Diabetes
Long C A, et al.
Annals of Surgery, 258(6) (2013)
Oligomerization of a molecular chaperone modulates its activity
Saio T, et al.
eLife, 7(6), e35731-e35731 (2018)
The C-terminal domain of Escherichia coli trigger factor represents the central module of its chaperone activity
Merz F, et al.
The Journal of Biological Chemistry, 281(42), 31963-31971 (2006)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) aggregation causes mitochondrial dysfunction during oxidative stress-induced cell death.
Nakajima H, et al.
The Journal of Biological Chemistry, 292(11), 4727-4742 (2017)
Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in DNA repair
Kosova A A, et al.
The Journal of Biological Chemistry, 82(6), 643-654 (2017)

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