G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

lyophilized powder, ≥75 units/mg protein

• Synonym(s): D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating), GAPDH
• CAS Number: 9001-50-7
• Enzyme Commission number: 1.2.1.12 (BRENDA, IUBMB)
• EC Number: 232-609-4
• MDL number: MFCD00131203
• NACRES: NA.54

Properties

• biological source: rabbit muscle
• Quality Level: 200
• form: lyophilized powder
• specific activity: ≥75 units/mg protein
• composition: Protein, ≥80% biuret
• foreign activity: 3-Phosphoglyceric phosphokinase ≤0.05%; lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%; triosephosphate isomerase ≤0.1%
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.

Application

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

• for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
• to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
• in anti-aggregation assays
• in purified GAPDH studies

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.

Unit Definition

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing citrate buffer salts

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves