All Photos(3)

G2500

Sigma-Aldrich

Gelatin from porcine skin

gel strength 300, Type A

CAS Number:
EC Number:
MDL number:
NACRES:
NA.61

Quality Level

biological source

Porcine skin

type

Type A

assay

70-90% (biuret)

form

powder

application(s)

cell culture | mammalian: suitable

gel strength

~300 g Bloom

solubility

H2O: soluble 50 mg/mL

Looking for similar products? Visit Product Comparison Guide

General description

Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources. It is a collagen derivative and comprises protein as a major component with minerals and water.

Application

Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Gelatin from porcine skin has been used:
  • for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay
  • for coating glass slides in the histological studies with brain sections
  • in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels
  • to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting

Packaging

1 kg in poly bottle
100, 500 g in poly bottle

Biochem/physiol Actions

Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture. It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry. It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Storage Class Code

11 - Combustible Solids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Gabriele Pitingolo et al.
Micromachines, 10(4) (2019-04-24)
Traditional two-dimensional (2D) cell culture models are limited in their ability to reproduce human structures and functions. On the contrary, three-dimensional (3D) microtissues have the potential to permit the development of new cell-based assays as advanced in vitro models to...
Nicola Contessi Negrini et al.
Acta biomaterialia, 87, 61-75 (2019-01-18)
When adipose tissue (AT) is impaired by trauma or disease, AT engineering could provide a shelf-ready structural and functional restoration as alternative to current clinical treatments, which mainly aim at aesthetic replacement. Yet, the lack of an efficient vascular network...
R F Vogt et al.
Journal of immunological methods, 101(1), 43-50 (1987-07-16)
We tested instantized dry milk, casein, gelatins from pig and fish skin, serum albumin and several other proteins for their abilities to block non-specific binding (NSB) of a peroxidase-conjugated immunoglobulin to polystyrene microtiter plate wells. Each blocking protein was tested...
J P Gobetti et al.
Journal of the American Dental Association (1939), 113(2), 291-292 (1986-08-01)
The study showed that washing a gloved hand removed significant amounts of bacteria. If the proper soap or scrub is used, the gloved hand will be free of bacteria. It is suggested that all dental personnel wear gloves to protect...
Kathrin S Heinz et al.
Nucleic acids research, 46(12), 6112-6128 (2018-05-12)
The replication of the genome is a highly organized process, both spatially and temporally. Although a lot is known on the composition of the basic replication machinery, how its activity is regulated is mostly unknown. Several chromatin properties have been...

Articles

Photo-Crosslinkable Gelatin Hydrogel: Versatile Materials for (High Resolution) Additive Manufacturing

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

Papain

Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Protocols

Trophoblast Protocol

This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service