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G2N10

Sigma-Aldrich

GenElute Plant Genomic DNA Miniprep Kit

greener alternative

sufficient for 10 purifications

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Synonym(s):
Plant Genomic DNA Miniprep, Gen Elute
NACRES:
NA.55

usage

sufficient for 10 purifications

Quality Level

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

15-25°C

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This Item
G2N70G1N10G2N350
greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

G2N350

G2N10, G2N350

G1N350, G1N70, G1N9602, G1N9604

G2N10, G2N70

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

-

greener alternative category

, Aligned

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

General description

The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute Plant Genomic DNA Miniprep Kit has been used:
  • in isolation of arbuscular mycorrhizal (AM) fungal spores DNA
  • in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA
  • in genomic DNA isolation from leaves

The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
  • PCR
  • restriction endonuclease digestion
  • cloning
  • Southern blots

Features and Benefits

  • Starting material: Up to 100 mg of plant tissue
  • Expected yield: Up to 20 μg
  • Elution volume: 100 - 200 μl
  • Time required: < 40 min
  • RNase treatment required: No

Principle

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation SolutionSDS

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Tae-Jin Kang et al.
BMC biotechnology, 4, 20-20 (2004-09-03)
DNA extraction methods for PCR-quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc.
Frida Meijer Carlsen et al.
Frontiers in genome editing, 3, 795644-795644 (2022-02-08)
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Santoshkumar M Shetty et al.
Journal of plant physiology, 169(7), 718-730 (2012-03-02)
The transcriptional regulation of multigenic eggplant (Solanum melongena) polyphenol oxidase genes (SmePPO) is orchestrated by their corresponding promoters which mediate developmentally regulated expression in response to myriad biotic and abiotic factors. However, information on structural features of SmePPO promoters and
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Fusarium verticillioides (teleomorph Gibberella moniliformis) is the main fungal agent of ear and kernel rot of maize (Zea mays L.) worldwide, including Italy. F.verticillioides is a highly toxigenic species since it is able to produce the carcinogenic mycotoxins fumonisins. In
Sabine Telle et al.
PloS one, 3(10), e3584-e3584 (2008-11-01)
During the past years an increasing number of studies have focussed on the use of herbarium specimens for molecular phylogenetic investigations and several comparative studies have been published. However, in the studies reported so far usually rather large amounts of

Protocols

This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service