This product is a crystalline powder, the pH is not determined on a lot-to-lot basis. The solubility of this material is tested at 573 g/L (6 M). Historically, the pH of the 6 Molar solution has ranged from 4.6 - 6.0 at 25 °C.
G3272
Guanidine hydrochloride
Molecular Biology, ≥99%
Guanidine hydrochloride
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Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 g | Available to ship TODAYfromMILWAUKEE | $58.30 | |
| 100 g | Available to ship TODAYfromMILWAUKEE | $107.00 | |
| 500 g | Available to ship TODAYfromMILWAUKEE | $345.00 | |
| 1 kg | Available to ship TODAYfromMILWAUKEE | $601.00 | |
| 2 kg | Available to ship TODAYfromMILWAUKEE | $1,070.00 | |
| 10 kg | Available to ship TODAYfromMILWAUKEE | Inquire |
About This Item
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
UNSPSC Code:
12352107
NACRES:
NA.31
PubChem Substance ID:
EC Number:
200-002-3
Beilstein/REAXYS Number:
3591990
MDL number:
$58.30
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biological source
synthetic (organic)
Quality Level
grade
Molecular Biology
assay
≥99%
form
crystalline powder
storage condition
(Tightly closed. Dry. )
technique(s)
RNA extraction: suitable
impurities
≤0.3% water (Karl Fischer)
color
white
pH
(25 °C, 4.6 - 6/573 g/L)
mp
180-185 °C (lit.)
solubility
H2O: 6 M, clear, colorless
density
1.3 g/cm3 (lit.)
cation traces
Pb: ≤5 ppm
UV absorption
λ: 260 nm Amax: ≤0.10, λ: 290 nm Amax: ≤0.05
application(s)
advanced drug delivery
foreign activity
DNase, RNase, none detected
SMILES string
Cl[H].NC(N)=N
InChI
1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
InChI key
PJJJBBJSCAKJQF-UHFFFAOYSA-N
Gene Information
human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)
General description
Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations.
At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.
At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research.
Application
Guanidine hydrochloride has been used:
- as a component of the extraction buffer for the extraction of proteoglycans[1]
- in extraction during protein fractionation of ATDC5 cell lines[2]
- as a chemical additive to study its effective absorbance spectra in structural analysis[3]
- to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer[4]
- as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study[5]
- in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Biochem/physiol Actions
Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule.[6][7] It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo.[6] It is a potent denaturant and inactivator of several enzymes and proteins.[7][8] GuHCl can inactivate aminoacylase and papain.[8] It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor.[8] At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).[9]
Features and Benefits
- Highly versatile surfactant for your cell biology and biochemical research
- Suitable for sensitive molecular biology applications
- Free from Exonuclease, Dnase and Rnase
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Preparation Note
In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Disclaimer
May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.
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This Item | |||
|---|---|---|---|
| technique(s) RNA extraction: suitable | technique(s) - | technique(s) - | technique(s) - |
| assay ≥99% | assay ≥98% | assay ≥99% | assay ≥99% (titration) |
| grade Molecular Biology | grade - | grade anhydrous | grade - |
| Quality Level 400 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form crystalline powder | form powder or crystals | form - | form solid |
| application(s) advanced drug delivery | application(s) - | application(s) - | application(s) - |
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocols
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Related Content
Yang Wei et al.
Biochimica et biophysica acta, 1844(12), 2331-2337 (2014-10-14)
Conventional empirical methods for the quantification of the helical content of proteins in solution using circular dichroism (CD) primarily rely on spectral data acquired between wavelengths of 190 and 230nm. The presence of chemical species in a protein solution with
Dafné Wilhelm et al.
Journal of proteomics, 219, 103718-103718 (2020-02-26)
Fibrillar collagens and proteoglycans (PGs) are quantitatively the major constituents of extracellular matrices (ECM). They carry numerous crucial post-translational modifications (PTMs) that tune the resulting biomechanical properties of the corresponding tissues. The mechanisms determining these PTMs remain largely unknown, notably
Ning Gao et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(51), 14633-14638 (2016-12-09)
Nanomaterial-based field-effect transistor (FET) sensors are capable of label-free real-time chemical and biological detection with high sensitivity and spatial resolution, although direct measurements in high-ionic-strength physiological solutions remain challenging due to the Debye screening effect. Recently, we demonstrated a general
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G3272-100G | 04061835544974 |
| G3272-25G | 04061835544998 |
| G3272-1KG | 04061835544981 |
| G3272-500G | 04061835545018 |
| G3272-2KG | 04061835545001 |
| G3272-10KG | 04065272302760 |
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
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For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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