G3512

Sigma-Aldrich

Glycerol Dehydrogenase from Cellulomonas sp.

lyophilized powder, 50-125 units/mg protein

Synonym(s):
Glycerol:NAD+ 2-oxidoreductase
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

50-125 units/mg protein

mol wt

~390 kDa

composition

Protein, ≥60% biuret

storage temp.

−20°C

General description

Glycerol Dehydrogenase belongs to iron-containing alcohol dehydrogenase family. It is made up of eight identical subunits which are about 42,000 Da each. The C-terminal has histidine and the N-terminal has serine amino acid.

Application

Glycerol Dehydrogenase (GDH) from Cellulomonas sp. has been used in the determination of glycerol by sequential injection analysis (SIA) method. It has also been used in the preparation of pyrroloquinoline quinone (PQQ) based biosensor for the determination of glycerol.
This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.

Packaging

250, 1000 units in poly bottle

Biochem/physiol Actions

Glycerol dehydrogenase catalyzes the conversion of glycerol to glycerone.

Physical properties

Isoelectric point : 4.4 ± 0.1
Michaelis constants : 1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
Structure : 10 subunits (42,000) per mol of enzyme
Inhibitors : p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal
ions (Co++, Ni++, Cu++, Zn++, Cd++)
Optimum pH : 10.0 – 10.5
Optimum temperature : 50°C
pH Stability : pH 7.5 – 10.5 (25°C, 20hr)
Thermal stability : below 55°C (pH 7.5, 15min)
Substrate specificity : This enzyme has the highest specificity for glycerol and 1,2-propanediol, and also oxidizes glycerol-α-monochlorohydrin, ethylene
glycol and 2,3-butanediol. The oxidative reaction is stimulated by K+, NH+4 and Rb+.

Unit Definition

One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25 °C at pH 10.0.

Physical form

Lyophilized powder containing bovine serum albumin

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A glycerol dehydrogenase from Escherichia coli
R.E. Asnis & A.F. Brodie
The Journal of Biological Chemistry, 23, 153-159 (1953)
An improved glycerol biosensor with an Au-FeS-NAD-glycerol-dehydrogenase anode
Mahadevan A and Fernando S
Biosensors And Bioelectronics, 92, 417-424 (2017)
Sequential injection kinetic flow assay for monitoring glycerol in a sugar fermentation process by Saccharomyces cerevisiae
Dominguez KBH, et al.
Applied Biochemistry and Biotechnology, 160(6), 1664-1673 (2010)
Glycerol dehydrogenase: structure, specificity, and mechanism of a family III polyol dehydrogenase
Ruzheinikov SN, et al.
Structure, 9(9), 789-802 (2001)
Ming-hua Li et al.
Bioresource technology, 101(21), 8294-8299 (2010-06-26)
Gluconobacter oxydans can rapidly and incompletely oxidize glycerol to dihydroxyacetone (DHA), a versatile product extensively used in cosmetic, chemical and pharmaceutical industries. To improve DHA production, the glycerol dehydrogenase (GDH) responsible for DHA formation was overexpressed in G. oxydans M5AM...
Protocols
Assay Procedure for Glycerol Dehydrogenase
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