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G4018

Sigma-Aldrich

Anti-Goat IgG (whole molecule) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:60,000
quantitative precipitin assay: 2.0 mg/mL

shipped in

dry ice

storage temp.

−20°C

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General description

The product binds to all goat Igs.
IgG is present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Immunogen

Goat IgG purified from a pool of normal goat serum.

Application

Anti-Goat IgG (whole molecule) antibody has been used in immunohistochemistry and in lateral flow strips (LF- strips).
Anti-Goat IgG (whole molecule) antibody produced in rabbit was used as control antibody in shear stress experiments conducted on porcine endothelial cells. It was used in ChIP assay to study the H19 imprinting control region in transgenic Drosophila.

Biochem/physiol Actions

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Preparation Note

Anti-Goat IgG (whole molecule)is an affinity isolated polyclonal antibody developed in rabbit using goat IgG purified from pooled goat serum as the immunogen. The antibody is purified to remove rabbit serum proteins including immunoglobulins which do not specifically bind to goat IgG.

Analysis Note

Each milliliter of product contains 2.0-2.5 mg of specific antibody measured by quantitative precipitation. Identity and purity is determined by immunoelectrophoresis(IEP).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Stefan Schoenfelder et al.
EMBO reports, 8(11), 1068-1073 (2007-10-20)
The imprinting control region (ICR) upstream of H19 is the key regulatory element conferring monoallelic expression on H19 and Igf2 (insulin-like growth factor 2). Epigenetic marks in the ICR regulate its interaction with the chromatin protein CCCTC-binding factor and with
Differential expression of RDC1/CXCR7 in the human placenta
Tripathi V, et al.
Journal of clinical immunology, 29(3), 379-379 (2009)
Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa
Corstjens P M,et al.
Clinical Biochemistry, 49(1-2), 22-31 (2016)
Márcio Pereira-da-Silva et al.
Endocrinology, 144(11), 4831-4840 (2003-09-10)
Short-term cold exposure of homeothermic animals leads to higher thermogenesis and food consumption accompanied by weight loss. An analysis of cDNA-macroarray was employed to identify candidate mRNA species that encode proteins involved in thermogenic adaptation to cold. A cDNA-macroarray analysis
Kidist Bobosha et al.
PLoS neglected tropical diseases, 8(5), e2845-e2845 (2014-05-09)
Field-applicable tests detecting asymptomatic Mycobacterium leprae (M. leprae) infection or predicting progression to leprosy, are urgently required. Since the outcome of M. leprae infection is determined by cellular- and humoral immunity, we aim to develop diagnostic tests detecting pro-/anti-inflammatory and

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