G4256

Sigma-Aldrich

Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast

Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)

Synonym(s):
UDP glucose:α-D-galactose-1-phosphate uridyltransferase, GALT
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54
Pricing and availability is not currently available.

Quality Level

type

Type IV

form

lyophilized powder

specific activity

20-60 units/mg protein (modified Warburg-Christian)

composition

Protein, 15-35%

foreign activity

6-phosphogluconate dehydrogenase ≤0.5%
UDP glucose pyrophosphorylase and galactokinase ≤0.2%

storage temp.

−20°C

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Packaging

20, 50 units in glass bottle

Biochem/physiol Actions

Galactose-1-phosphate uridyltransferase (GALT) facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity.

Unit Definition

One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase.

Physical form

Contains buffer salts as citrate and reduced glutathione

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Daniel Lazarevic et al.
Carbohydrate research, 344(12), 1449-1452 (2009-06-30)
All monodeoxygenated galactoses were treated with galactokinase, and for the 2-, 3-, and 4-deoxy compounds, transformation into the corresponding galactopyranosyl phosphates could be observed. In case of the 2-deoxy derivative, further reaction via UDP-2-deoxy-D-lyxo-hexose (UDP-2-deoxygalactose), which was also obtained chemically...
Nir Dai et al.
Plant physiology, 142(1), 294-304 (2006-07-11)
The Cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose. These are initially hydrolyzed by alpha-galactosidase to yield free galactose (Gal) and, accordingly, Gal metabolism is an important pathway in Cucurbitaceae...
E Crushell et al.
Journal of inherited metabolic disease, 32(3), 412-415 (2009-05-07)
Classical galactosaemia is relatively common in Ireland due to a high carrier rate of the Q188R GALT mutation. It is screened for using a bacterial inhibition assay (BIA) for free galactose. A Beutler assay on day one of life is...
Jason G McCoy et al.
Biochemistry, 45(10), 3154-3162 (2006-03-08)
The X-ray crystal structure of the At5g18200.1 protein has been determined to a nominal resolution of 2.30 A. The structure has a histidine triad (HIT)-like fold containing two distinct HIT-like motifs. The sequence of At5g18200.1 indicates a distant family relationship...
Mohamed Jama et al.
The Journal of molecular diagnostics : JMD, 9(5), 618-623 (2007-09-22)
Classic galactosemia is an autosomal recessive inherited error of galactose metabolism. It is caused by lack of galactose-1-phosphate uridyl transferase, an enzyme that is required to metabolize galactose-1-phosphate to uridine diphosphate galactose. The build up of galactose-1-phosphate is toxic at...

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