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G5003

Sigma-Aldrich

α-Glucosidase from Saccharomyces cerevisiae

greener alternative

Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

Synonym(s):
α-D-Glucosidase, α-D-Glucoside glucohydrolase, Maltase from yeast
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type I

Quality Level

form

lyophilized powder

specific activity

≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

contains

phosphate buffer salts and EDTA as balance

composition

Protein, ≥50%

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

UniProt accession no.

foreign activity

β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%

greener alternative category

relevant disease(s)

diabetes (management of non-insulin-dependent diabetes mellitus)

storage temp.

−20°C

Gene Information

bakers yeast ... MAL12(853209) , MAL32(852602)

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Application

α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.
For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters

Packaging

100 units in glass bottle
1000 units in poly bottle
Sold on basis of p-nitrophenyl α-D-glucoside units.

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.
Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.

Unit Definition

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Analysis Note

Protein determined by biuret.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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